拟南芥黄心突变体yh基因的图位克隆及分析  

Map-based Cloning and Analysis of the yh Gene in Arabidopsis thaliana Yellow Heart Mutant

作　　者：郝亚琦[1] 宗秀梅 任潘 付爱根 HAO Yaqi;ZONG Xiumei;REN Pan;FU Aigen(Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education,Provincial Key Laboratory of Biotechnology of Shaanxi Province,College of Life Sciences,Northwest University,Xi'an,China,710069)

机构地区：[1]教育部西部资源生物与现代生物技术教育部重点实验室,陕西省生物技术与生化工程重点实验室,西北大学生命科学学院,西安710069

出　　处：《西北植物学报》2021年第10期1621-1626,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金青年基金(31300204);陕西省科技厅青年基金(2019JQ-326)。

摘　　要：在研究光合作用相关基因的过程中,获得了一个叶片为黄心(yellow heart,yh)的突变株,与野生型拟南芥(Col-0)相比,其新生叶片发黄,突变表型由隐性单基因控制。采用图位克隆及其精细定位技术,将yh突变基因定位在1号染色体的INS1_55_342与INS1_56_34区间,物理距离约为676 kb。通过测序得知yh在At1g64790第44个内含子剪接处有4个碱基的缺失,导致内含子剪切位点的变化。RT-PCR分析显示,该基因表达降低,是At1g64790基因的一个新等位突变。研究表明,yh突变体与叶绿体的发育相关,可为进一步探究植物叶绿体和叶片发育机制提供新的遗传材料。In the process of investigating the photosynthesis related genes,we obtained a recessive Arabidopsis thaliana mutant,yellow heart(yh).Compared with the wild type(Col-0)A.thaliana,yh mutant appeared a yellow heart phenotype in new leaves.With map-based cloning strategy,the mutant gene yh was precisely mapped into the 676 kb chromosome interval between the molecular markers INS1_55_342 and INS1_56_34 on chromosome 1.The yh mutant has a 4 base deletion at the splice junction of the 44th intron,which result in a change in the splice site of the intron.RT-PCR results have indicated that the expression of yh gene is knock down,and yh mutant is indeed a new allelic mutation in gene At1g64790.Based on study above,yh mutant may provide a new genetic material to explore the chloroplast and leaf development mechanisms.

关 键 词：图位克隆 内含子剪接 叶色 叶发育 

分 类 号：Q343.17[生物学—遗传学] Q786