吸水和干燥条件下发菜核糖体代谢差异表达基因分析  

Differential Expression Genes of Ribosomal Metabolism in Nostoc flagelliforme Subjected to Hydration and Dehydration

作　　者：胡进红 马晓蓉 宋繁 梁旺利 梁文裕[1] 王玲霞[1] HU Jinhong;MA Xiaorong;SONG Fan;LIANG Wangli;LIANG Wenyu;WANG Lingxia(School of Life Sciences, Ningxia University, Yinchuan 750021, China)

机构地区：[1]宁夏大学生命科学学院,银川750021

出　　处：《西北植物学报》2021年第10期1643-1651,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：第四批宁夏青年科技人才托举工程(NXTJ2019006);宁夏回族自治区科技创新团队建设项目(NXKJT2019002)。

摘　　要：为解析发菜吸水和干燥条件下的基因差异表达规律以及发菜适应“干-湿”水分节律调控机制,该研究以充分吸水发菜藻体为对照组,干燥状态下的发菜藻体为处理组,采用高通量Illumina HiSeq PE150测序平台结合生物信息学分析方法对发菜吸水和干燥条件下的差异表达基因进行分析。结果显示:(1)发菜吸水和干燥条件下共鉴定到差异表达基因3383个,其中显著上调和显著下调表达的基因数分别为1767个和1616个。(2)GO功能富集分析发现,在吸水和干燥条件下发菜差异表达基因主要富集在蛋白质合成与代谢等相关途径中;KEGG显著性富集分析发现,吸水和干燥条件下发菜有46个差异表达基因被显著富集到核糖体代谢途径,且均为显著上调表达,这些基因可能参与发菜对干旱胁迫的响应。(3)随机挑选6个被显著富集到核糖体代谢途径的基因进行qRT-PCR分析发现,其基因相对表达量与转录组测序结果一致。研究表明,发菜在干燥条件下可能通过激活核糖体代谢特定基因表达帮助抗旱相关的蛋白质的合成和正确折叠、并参与渗透调节等重要生理活动进而调控其适应干燥环境条件。In order to analyze the variation of differentially expressed genes and the regulation mechanism of“dry-wet”adaptation in N.flagelliforme under hydration and dehydration.High-throughput Illumina HiSeq PE150 sequencing platform and bioinformatics method were used to screen the drought-tolerant differentially expressed genes of N.flagelliforme in this study.The results showed that:(1)a total of 3383 differentially expressed genes were identified under hydration and dehydration,among which the significantly up-regulated and down-regulated genes were 1767 and 1616,respectively.(2)GO function enrichment analysis showed that the expression and regulation of differentially expressed genes mainly focused on protein synthesis and metabolism pathway in N.flagelliforme under dehydration.The KEGG significant enrichment analysis showed that the 46 differentially expressed genes in N.flagelliforme under hydration and dehydration were significantly enriched to the ribosomal metabolic pathway,all of which were up-regulated.(3)Six genes that were significantly enriched in the ribosomal metabolic pathway were randomly selected for qRT-PCR analysis,and the gene expression levels were consistent with the transcriptome sequencing results.These results suggested that N.flagelliforme might adapt to drought stress by inducing the gene expressions of ribosomal metabolic pathway in order to promoting drought-related protein synthesis and correct folding.These proteins involved in osmotic regulation and other important physiological activities in N.flagelliforme under dehydration.

关 键 词：发菜 吸水和干燥 差异表达基因 核糖体蛋白 

分 类 号：Q786[生物学—分子生物学]