不同浓度Fe^(2+)与Zn^(2+)对羊草幼苗生长和生理特性的影响  

Effect of Different Concentrations of Fe^(2+)and Zn^(2+)on Growth and Physiological Characteristics of Leymus chinensis Seedlings

作　　者：孟令博 赵曼 亢燕[1] 祁智[1] MENG Lingbo;ZHAO Man;KANG Yan;QI Zhi(College of Life Sciences, Key Laboratory of Herbage and Endemic Crop Biology, Ministry of Education,Inner Mongolia University, Hohhot 010031, China)

机构地区：[1]内蒙古大学生命科学学院/牧草与特色作物生物学教育部重点实验室,呼和浩特010031

出　　处：《西北植物学报》2021年第10期1681-1690,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：内蒙古自治区科技重大专项(zdzx2018016);国家自然科学基金(32060063)。

摘　　要：以羊草幼苗为研究对象,通过调整全营养培养基(CK,0.05 mmol/L Fe^(2+)、0.015 mmol/L Zn^(2+))中铁或者锌含量设置0、10倍、20倍Fe^(2+)(Zn^(2+))浓度处理Fe_(0)(Zn_(0))、Fe_(10)(Zn_(10))、Fe_(20)(Zn_(20)),以及在高铁培养基中单独添加0.15 mmol/L Zn^(2+)或同时添加10 mmol/L Ca^(2+)、5 mmol/L Mg^(2+)、20 mmol/L K^(+)处理,测定培养6 d后幼苗生长指标和矿质元素含量、以及高铁(Fe_(20))处理下幼苗根中抗氧化指标和相关基因表达量,探究不同浓度Fe^(2+)、Zn^(2+)对羊草幼苗生长、矿质元素吸收积累及抗氧化指标、基因表达的影响。结果表明:(1)缺锌(Zn0)显著抑制羊草幼苗鲜重的增加和Zn元素的积累,但促进Fe、Mg元素的积累;高浓度锌(Zn_(10)、Zn_(20))显著促进幼苗叶片生长和Zn元素的积累;缺铁(Fe0)显著抑制幼苗的根长、鲜重和Fe元素的积累,促进Mg、Zn元素的积累;高浓度铁(Fe_(10)、Fe_(20))显著抑制羊草幼苗根叶生长、根毛发育和Ca、Zn、Mg、K元素的积累。(2)增加Zn^(2+)和Ca^(2+)、Mg^(2+)、K^(+)浓度无法恢复高铁胁迫对幼苗生长的抑制作用。(3)高浓度铁(Fe_(20))处理羊草幼苗48 h后,根部过氧化物酶、超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶、谷胱甘肽还原酶活性和丙二醛、抗坏血酸、还原型谷胱甘肽含量显著升高;烟酰胺合成酶基因、过氧化物酶基因表达量显著下调,植物类萌发素蛋白基因表达量显著上调。研究发现,羊草幼苗生长发育和矿质元素积累对环境中Zn^(2+)浓度变化不敏感,却受到环境中高浓度Fe^(2+)的显著抑制,并造成严重的氧化胁迫伤害,这种伤害无法在添加Zn^(2+)或同时添加Ca^(2+)、Mg^(2+)、K^(+)的条件下恢复。With Leymus chinensis seedlings as the research object,we set 0,10 and 20 times Fe^(2+)(Zn^(2+))concentration by adjusting the contents of iron and zinc in total nutrition medium(CK:0.05 mmol/L Fe^(2+),0.015 mmol/L Zn^(2+))as Fe_(0)(Zn_(0)),Fe_(10)(Zn_(10)),Fe_(20)(Zn_(20)).And adding 0.15 mmol/L Zn^(2+)alone or 10 mmol/L Ca^(2+),5 mmol/L Mg^(2+),20 mmol/L K^(+)to the high concentration iron medium at the same time.We measured the growth index and mineral element contents of seedlings after 6 days of culture,as well as the antioxidant indexes and related gene expression in the roots of seedlings treated with high concentration iron(Fe_(20)),to explore the effects of different concentrations of Fe^(2+)and Zn^(2+)on the growth,mineral element absorption and accumulation,antioxidant indexes and gene expression in Leymus chinensis seedlings.The results show that:(1)deficiency of Zn(Zn_(0))significantly inhibited the increase of fresh weight and the accumulation of Zn elements,but promoted the accumulation of Fe and Mg elements.High concentration of Zn(Zn_(10),Zn_(20))significantly promoted the growth of seedling leaves and the accumulation of Zn.Deficiency of Fe(Fe_(0))significantly inhibited the root length,fresh weight and the accumulation of Fe elements,and promoted the accumulation of Mg and Zn elements in seedlings.High concentration of Fe(Fe_(10),Fe_(20))significantly inhibited the root and leaf growth,root hair development and the accumulation of Ca,Zn,Mg and K elements in L.chinensis seedlings.(2)Increasing the concentration of Zn^(2+)and Ca^(2+),Mg^(2+),K^(+)could not restore the inhibitory effect of high concentration iron stress on seedling growth.(3)After treatment of L.chinensis seedlings with high concentration of iron(Fe_(20))for 48 hours,the activities of peroxidase,superoxide dismutase,catalase,ascorbate peroxidase,glutathione reductase and the contents of malondialdehyde,ascorbic acid and reduced glutathione in roots increased significantly,while the gene expressions of nicotianamine synthase and pe

关 键 词：羊草 铁 锌 生理特性 

分 类 号：Q945.78[生物学—植物学]