紫甘薯叶片响应UV-B辐射增强的转录组分析  

Transcriptome Analysis of Purple Sweet Potato Leaf in Response to Enhanced UV-B Radiation

作　　者：孟凡来[1,2] 白磊 郭华春 赵大伟[2] 余兴华 王应梅 李玉祥 滕娟 MENG Fanlai;BAI Lei;GUO Huachun;ZHAO Dawei;YU Xinghua;WANG Yingmei;LI Yuxiang;TENG Juan(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;Wenshan Zhuang and Miao Autonomous Prefecture Academy of Agricultural Sciences,Wenshan 663099,China)

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]文山壮族苗族自治州农业科学院,云南文山663099

出　　处：《华北农学报》2021年第5期135-142,共8页Acta Agriculturae Boreali-Sinica

基　　金：国家农业产业技术体系(CARS-09-15P)。

摘　　要：为了研究紫甘薯叶片响应UV-B辐射增强的分子机理,以滇紫甘薯24(DZS24)的叶片为试验材料,通过高通量测序技术对自然光照与人工增补UV-B辐射(T=7.2 kJ/(m 2·d))下DZS24的叶片进行转录组测序分析。结果表明,477个基因表达发生显著变化,其中382个上调表达,95个下调表达,GO功能分析显示,差异表达基因主要显著富集在生物过程的氧化还原过程和分子功能的氧化还原酶活性中,KEGG代谢通路分析共富集到9条显著差异通路,其中次生代谢产物生物合成通路所包含的差异表达基因数量最多。紫甘薯叶片响应UV-B辐射增强的差异表达基因主要为类黄酮合成通路中的CHS(Tai6.1732、Tai6.53503、Tai6.45381)和DFR(Tai6.3019)及芥子油苷生物合成中的CYP83B1(Tai6.44115、Tai6.18064、Tai6.41206)和油菜素内酯生物合成中的CYP85A1(Tai6.2806、Tai6.19253、Tai6.15801),转录因子主要为C2H2、NAM、bHLH和RR-A-type。综上所述,紫甘薯叶片响应UV-B辐射增强的关键基因可能为IbCHS、IbDFR、IbCYP83B1和IbCYP85A1,主要转录因子可能为C2H2、NAM、bHLH和RR-A-type。In order to study the molecular mechanism of purple sweet potato leaf in response to enhanced UV-B radiation,the leaf of Dianziganshu 24(DZS24)was used as the test material and transcriptome sequencing analysis was performed by high-throughput sequencing technology under natural light and artificial supplementary the UV-B(T=7.2 kJ/(m 2·d)).The results showed that the expression of 477 genes(DEGs)were changed significantly,among which 382 and 95 were up-and down-regulated ones.GO analysis showed that these DEGs were mainly significantly enriched in oxidation-reduction process of biological process and oxidoreductase activity of molecular function.KEGG pathway analysis showed that a total of 9 pathways were significantly enriched,among which the biosynthesis pathway of secondary metabolites contained the largest number of DEGs.The DEGs of purple sweet potato leaf in response to enhanced the UV-B were mainly CHS(Tai6.1732,Tai6.53503,Tai6.45381)and DFR(Tai6.3019)in the flavonoid synthesis pathway as well as the CYP83B1(Tai6.44115,Tai6.18064,Tai6.41206)in the glucosinolate biosynthesis and CYP85A1(Tai6.2806,Tai6.19253,Tai6.15801)in brassinosteroid biosynthesis.The main transcription factors were C2H2,NAM,bHLH and RR-A-type.In summary,the key genes might be IbCHS,IbDFR,IbCYP83B1 and IbCYP85A1,as well as the main transcription factors might be C2H2,NAM,bHLH and RR-A-type in the purple sweet potato leaf response to the UV-B enhancement.

关 键 词：紫甘薯叶片 UV-B辐射增强 差异表达基因 响应 

分 类 号：S531.03[农业科学—作物学] Q78[生物学—分子生物学]