荷兰鸢尾突变株‘紫韵’的花色突变相关机理分析  

Analysis of Related Genes about Flower Color Mutation in‘Ziyun’of Iris hollandica

作　　者：林兵[1] 陈艺荃 方能炎 钟淮钦[1] 叶秀仙[1] 樊荣辉[1] LIN Bing;CHEN Yiquan;FANG Nengyan;ZHONG Huaiqin;YE Xiuxian;FAN Ronghui(Institute of Crop Sciences, Fujian Academy of Agricultural Science, Fuzhou 350013, China;2Institute of Agriculture and Engineering Technology, Fujian Academy of Agricultural Science, Fuzhou 350013, China)

机构地区：[1]福建省农业科学院作物研究所,福州350013 [2]福建省农业科学院农业工程技术研究所,福州350013

出　　处：《西北植物学报》2021年第9期1509-1515,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：福建省属公益类科研专项(2018R1026-6);福建省农业科学院科技创新团队项目(CXTD2021039)。

摘　　要：为了探索荷兰鸢尾蓝紫色花及突变紫色花的显色分子机制及色素沉积差异,该研究以蓝紫色野生型‘展翅’和紫色突变株‘紫韵’为材料,通过花色素苷测定、转录组测序和qRT-PCR方法对花色变异进行分析。结果表明:(1)紫色突变株‘紫韵’花旗瓣中的总花色素苷含量(392.7μg·g^(-1))显著低于野生型‘展翅’(543.5μg·g^(-1));与‘展翅’相比,‘紫韵’有3种花色素苷含量显著下降[矢车菊素-3-芸香糖苷含量由144.42μg·g^(-1)降为46.39μg·g^(-1),矮牵牛素-3-(6-鼠李糖基-2-木糖基葡萄糖苷)含量由61.86μg·g^(-1)降为31.67μg·g^(-1),矢车菊素-3-(2G-木糖基芸香糖苷)含量由25.22μg·g^(-1)降为7.65μg·g^(-1)],但6-羟基矢车菊素-3-葡萄糖苷含量由5.88μg·g^(-1)升为10.34μg·g^(-1)。(2)RNA-seq分析共获得46530个unigenes,与‘展翅’相比,‘紫韵’有43个基因上调表达,73个基因下调表达;层次聚类分析发现,花色素苷途径中共有2个差异表达基因——查尔酮合成酶(CHS)基因(IhCHS1)和花青素3-O葡萄糖转移酶(UFGT)基因(IhUFGT1),且二者均下调表达。(3)qRT-PCR分析表明,随着花的发育,IhUFGT1在2个品种中表达量均上升,始花期达到最高,且在‘紫韵’花中的表达明显低于‘展翅’。研究认为,4种花色素苷含量的显著变化,可能是导致花色由野生型‘展翅’蓝紫色向突变株‘紫韵’紫色方向转变的主要原因;IhUFGT1基因在紫色‘紫韵’花中表达量比‘展翅’相对大幅度的降低,致使花色素苷含量相应变化,最终导致花色由蓝紫色转为紫色。Iris hollandica is an important ornamental bulbous plant.In this study,to elucidate molecular mechanism of coloration,as well as anthocyanin accumulation in blue and mutant purple flowers,we used blue wild type‘Zhanchi’and purple mutant‘Ziyun’as materials to analyze flower color variation by anthocyanin determination,transcriptome sequencing and qRT-PCR.The results show that:(1)the total anthocyanin content of purple mutant‘Ziyun’in inner tepals(392.7μg·g^(-1))was significantly lower than that of wild type‘Zhanchi’(543.5μg·g^(-1)).Compared with‘Zhanchi’,the content of three anthocyanins in‘Ziyun’significantly decreased[The content of cyanidin-3-rutinoside decreased from 144.42 to 46.39μg·g^(-1).The content of petuniacin-3-(6-rhamnosyl-2-xylosylglucoside)decreased from 61.86 to 31.67μg·g^(-1).The content of Cyanidin-3-(2G-xylosylrutinoside)decreased from 25.22 to 7.65μg·g^(-1)].However,the content of 6-hydroxycyanidin-3-glucoside increased from 5.88 to 10.34μg·g^(-1).(2)RNA-seq analysis revealed that a total of 46530 unigenes were obtained,compared with‘Zhanchi’,‘Ziyun’had 43 up-regulated genes and 73 down-regulated genes.Hierarchical cluster analysis showed that there were two differentially expressed genes in the anthocyanin pathway:chalcone synthase(CHS)gene(IhCHS1)and anthocyanin 3-O glucose transferase(UFGT)gene(IhUFGT1),and both of them were down regulated.(3)qRT-PCR showed that,the expression of IhUFGT1 increased in both varieties with the development of flowers,and reached the highest in early flowering period,and the expression in‘Ziyun’flower was significantly lower than that in‘Zhanchi’.According to the study,significant change of four anthocyanin content may be the main reason for flower color change from wild type‘Zhanchi’to mutant‘Ziyun’.The expression level of IhUFGT1 in purple‘Ziyun’flower was significantly lower than that in‘Zhanchi’,which resulted in the change of anthocyanin content,and finally led to the change of flower color

关 键 词：荷兰鸢尾 花色素苷 转录组分析 花青素3-O葡萄糖转移酶基因 

分 类 号：Q946.83[生物学—植物学] Q786