米槁组织培养不定芽发生与继代增殖  

Induction of Adventitious Buds and Subsequent Proliferation in Tissue Culture of Cinnamomum migao

作　　者：梁格林 刘济明[1,2] 武梦瑶 刘欢 唐子燕 LIANG Gelin;LIU Jiming;WU Mengyao;LIU Huan;TANG Ziyan(College of Forestry, Guizhou University, Guiyang 550025, China;Forest Ecology Research Center, Guizhou University, Guiyang 550025, China)

机构地区：[1]贵州大学林学院,贵阳550025 [2]贵州大学森林生态研究中心,贵阳550025

出　　处：《西北植物学报》2021年第9期1552-1558,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：贵州省科技计划(黔科合支撑[2019]2774号);黔南州社会发展科技计划。

摘　　要：该研究以米槁的带腋芽茎段为实验材料,建立米槁不定芽诱导及增殖培养体系,探究不同消毒时间、取材时间及不同激素种类和浓度配比条件对米槁带芽茎段不定芽诱导及增殖的影响。结果表明:(1)外植体最适消毒方式为75%酒精30 s+0.1%HgCl26.5 min,11月为外植体取材进行消毒的最佳时期。(2)最适不定芽诱导培养基为MS+3.0 mg/L 6-BA+0.3 mg/L IBA+6.5 g/L琼脂+30 g/L蔗糖,不定芽诱导率可达到81.24%;6-BA和NAA均能促进米槁不定芽的增殖,且NAA是不定芽增殖的主要影响因素。(3)最适不定芽增殖培养基为MS+0.1 mg/L 6-BA+1.0 mg/L NAA+0.3 g/L AC+6.5 g/L琼脂+30 g/L蔗糖,不定芽平均增殖系数达到2.79。该研究基本建立了米槁不定芽诱导及增殖培养体系,为其深层次的资源开发、利用及快速繁殖提供了一条新的路径。In this study,the stems of Cinnamomum migao with axillary buds were used as experimental materials to establish an induction and multiplication culture system for C.migao's adventitious buds.It was explored that the influence of adventitious bud induction and proliferation of C.migao stems with buds under the conditions of different disinfection time,sampling time,and different hormone types and concentration ratios.The results showed that:(1)the most suitable disinfection method for explants was 75%alcohol for 30 s+0.1%HgCl2 for 6.5 min,and November was the best time for explants to be disinfected.(2)The most suitable adventitious bud induction medium is MS+3.0 mg/L 6-BA+0.3 mg/L IBA+6.5 g/L agar+30 g/L sucrose,the adventitious bud induction rate reaches 81.24%;both 6-BA and NAA can promote C.migao for the proliferation of adventitious buds,NAA is the main factor influencing the proliferation of adventitious buds.(3)The most suitable adventitious bud multiplication medium is MS+0.1 mg/L 6-BA+1.0 mg/L NAA+0.3 g/L AC+6.5 g/L agar+30 g/L sucrose,and the average adventitious bud multiplication coefficient reaches 2.79.This study basically established the adventitious bud induction and multiplication culture system of C.migao,which provides a new way for its in-depth resource development and utilization.

关 键 词：米槁 组织培养 不定芽 诱导与增殖 

分 类 号：Q813.13[生物学—生物工程]