云南景迈古茶园茶叶内生真菌多样性初步研究  

Preliminary study of endophytic fungus diversity of tea leaf in an ancient tea garden,Jingmai,Yunnan,China

作　　者：张建坤[1] 周剑 彭超[3] 王琴 汪飞 王振华 ZHANG Jian-kun;ZHOU Jian;PENG Chao;WANG Qin;WANG Fei;WANG Zhen-hua(Wuhan University of Technology,Wuhan 430070,China;Simao Customs Comprehensive Technical Service Center,Puer 665000,Yunnan,China;China Quality Certification Centre,Wuhan Branch,Wuhan 430062,China;Wuhan Customs Technology Center,Wuhan 430054,China)

机构地区：[1]武汉理工大学,武汉430070 [2]思茅海关综合技术服务中心,云南普洱665000 [3]中国质量认证中心武汉分中心,武汉430062 [4]武汉海关技术中心,武汉430054

出　　处：《湖北农业科学》2021年第19期130-134,共5页Hubei Agricultural Sciences

基　　金：海关总署科技计划项目(2017IK247;2020HK175)。

摘　　要：采集云南景迈和芒景古茶园的茶叶样品,经75%乙醇和0.5%次氯酸钠表面消毒后接种在PDA平板上,以分离自然干燥茶叶的内生真菌。共获得103个真菌纯培养物,根据形态学特征挑选30个分离物进行rRNA基因ITS区域和TEF基因的PCR扩增和测序。结果表明,30个分离物属于2门3纲7目10科13属,其中鬼伞菌(Coprinellus spp.)分离物最多,占20.0%,多节孢菌(Nodulisporium spp.)、炭团菌(Hypoxylon spp.)和轮层炭菌(Daldinia spp.)依次排在其后,分别占16.7%、13.3%和10.0%。与已报道的文献相比,本研究为茶叶内生真菌增加了1目4科8属的分类单位,多个分离物(如6、10、18、21、24、26)极可能是真菌新种。云南景迈茶园不同于一般的人工栽培茶园,其内生真菌种类可能极其丰富、独特,值得进一步调查。Tea leaf samples were collected from an ancient tea garden in Jingmai and Mangjing,Yunnan,China,and the naturally dried tea leaves were disinfected using 75%alcohol and 0.5%sodium hypochlorite and inoculated in PDA(Potato Dextrose Agar)plates for isolation of endophytic fungi.103 fungal isolates were obtained,and basing on the morphological features 30 were selected for PCR and sequencing of ITS region and TEF gene.The results showed that 30 isolates were classified into 2 phyla,3 classes,7 or⁃ders,10 families,and 13 genera,in which the isolates of Coprinellus spp.were the most(20.0%),and Nodulisporium spp.(16.7%),Hypoxylon spp.(13.3%),and Daldinia spp.(10.0%)were behind in order.Comparing with reported references,the isolates in this study added taxonomic units of one order,four families and eight genera to the endophytic fungi in tea plant.Several isolates(such as 6,10,18,21,24,26)were extremely possibly novel species of fungi.This study supposed that the ancient tea garden of Jingmai,Yunnan was quite different from usual artificially cultured tea gardens,and its endophytic fungi were extremely diverse and unique and deserved further investigation.

关 键 词：茶树 内生真菌 ITS 多样性 

分 类 号：Q819[生物学—生物工程]