凝胶色谱法(GPC)检测特殊膳食食品中小分子肽的分子量分布的研究  

Research on Molecular Weight Distribution of Small Molecule Peptides in Special Dietary Foods in Gel Chromatography

作　　者：黄伟乾 霍玟希 罗浩[1] 何敏恒 梁志森 覃天福 邱俊杰 HUANG Weiqian;HUO Wenxi;LUO Hao;HE Minheng;LIANG Zhisen;Qin Tianfu;QIU Junjie(China National Analytical Center(Guangzhou),Guangzhou 511447,China;Guangzhou Inspection Testing and Certification Group Co.,Ltd.,Guangzhou 511447,China)

机构地区：[1]国家加工食品质量检验中心(广东),广东广州511447 [2]广州检验检测认证集团有限公司,广东广州511447

出　　处：《现代食品》2021年第17期192-195,共4页Modern Food

基　　金：广州市场监督管理局科技项目(2020kj47)。

摘　　要：本文建立了特殊膳食食品中小分肽的分子量分布的凝胶色谱测定方法。以流动相进行样品的溶解提取,采用串联TSK-GEL凝胶色谱柱分离,以乙腈+0.06%三氟乙酸溶液(10+90)为流动相。在优化的条件下,采用0.7 mL·min^(-1)的速度进行等度洗脱,以6种分子量标记物进行分子量分布区间标识,相对分子量在121~12500 Da,相对分子量的对数量与保留时间成呈线关系(R^(2)为0.9495)。该方法前处理简单,可用于特殊膳食食品中肽分子量分布的检测。A method for the determination of molecular weight distribution of small peptides in special dietary foods by gel permeation chromatography was established.The mobile phase was used for the dissolution and extraction of samples.It is separated by a series of TSK-GEL gel chromatography columns,with acetonitrile+0.06%trifluoroacetic acid solution(10+90)as the mobile phase.Under the optimized conditions,0.7 mL·min^(-1) isometric elution was used to identify the molecular weight distribution of 6 molecular weight markers,with a relative molecular weight of 121~12500 Da.There was a linear relationship between the relative molecular weight and the retention time(R^(2)=0.9495).The method has simple pretreatment and can be used to detect the molecular weight distribution of peptides in special dietary foods.

关 键 词：凝胶色谱 特殊膳食 肽 分子量 

分 类 号：O657.7[理学—分析化学]