木薯根域AMF群落结构特征及多样性研究  

Study on the Structural Characteristics and Diversity of AMF Community in Cassava Root Zone

作　　者：周时艺 韦云东 陈蕊蕊 郑华[1] 李军[1] 盘欢[1] 罗燕春[1] ZHOU Shiyi;WEI Yundong;CHEN Ruirui;ZHENG Hua;LI Jun;PAN Huan;LUO Yanchun(Guangxi Subtropical Crops Research Institute,Nanning,Guangxi 530001,China)

机构地区：[1]广西壮族自治区亚热带作物研究所,广西南宁530001

出　　处：《农业研究与应用》2021年第4期54-61,共8页Agricultural Research and Application

基　　金：现代农业产业技术体系建设专项资金资助(NO.CARS-11);广西自然科学基金资助(No.2020GXNSFAA259042);广西农业科学院基本科研业务专项资助项目(桂农科2021YT150)。

摘　　要：土壤微生物是土壤—作物系统养分循环的重要驱动力,其中丛枝菌根真菌(Arbuscular mycorrhizal fungi,AMF)能够促进作物对养分的吸收。木薯是我国南方重要的经济作物,是多种行业的重要原材料。为揭示不同土壤质地木薯根域土壤AMF群落结构特征,解析土壤因子对AMF类群的影响。以木薯品种华南205(SC205)为材料,通过田间根袋培养,利用高通量测序平台进行真菌扩增子测序,分析不同处理木薯根域AMF类群组成和多样性差异。结果表明,木薯根域土壤AMF主要为球囊霉属(Glomus)、无梗囊霉属(Acaulospora)、盾巨孢囊霉属(Scutellospora)、类球囊霉属(Paraglomus)和双型囊霉属(Ambispora)。优势属为球囊霉属和无梗囊霉属。两种土质各处理AMF属水平上无显著差异,种水平分布受土壤质地及根袋的影响,粘土AMF多样性(Simpson指数)高于砂质壤土。两种土质中都发现独有种,土壤质地对木薯根域AMF群落分布有一定的影响。RDA分析表明Glomus与速效磷、速效氮、铵态氮正相关;Acaulospora与速效磷,速效氮弱负相关;Scutellospora与所有速效养分负相关;Paraglomus与所有速效养分表现出正相关;Ambispora与大部分速效养分表现出负相关。木薯根域AMF资源丰富,不同土壤质地条件下的AMF群落组成和丰度存在差异,土壤速效养分与AMF群落结构差异密切相关。该研究为进一步探索AMF在木薯上的应用提供理论参考。Soil microorganism is an important driving force of nutrient cycling in soil-crop system,and arbuscular mycorrhizal fungi(AMF)can increase the nutrient absorption of crops.Cassava is an important cash crop in the south of China,and it is an important raw material for many industries.In order to reveal the AMF community structure characteristics in cassava root zone with different soil textures and analyze the effects of soil factors on AMF groups,a root-bag experiment was set with cassava variety SC205 as material.We analyzed the composition and diversity of AMF groups in the cassava root zone under different treatments via high-throughput sequencing.The results showed that the main AMF of cassava root soil were Glomus,Acaulospora,Scutellospora,Paraglomus and Ambispora,and the dominant groups were Glomus and Acaulospora.There was no significant difference at AMF genera level between two soil treatments.The distribution of species level was affected by soil texture and root-bag,and Simpson index of clay was higher than sandy loam.Unique species were found in both soils,and soil texture had a certain influence on the distribution of AMF community in cassava root zone.RDA analysis showed that Glomus was positively correlated with available P,available N and ammonium N;Acaulospora was negatively correlated with available P and available N;Scutellospora was negatively correlated with all available nutrients;Paraglomus was positively correlated with all available nutrients.Ambispora was negatively correlated with most available nutrients.The cassava root zone soil is rich in AMF resources,the AMF community composition and abundance differed in different soil textures,and soil available nutrients and soil structure are closely related to AMF community.This study provides a theoretical reference for further exploration of AMF application in cassava production.

关 键 词：木薯 丛枝菌根真菌 高通量测序 球囊霉属 多样性 

分 类 号：S154.3[农业科学—土壤学] S533[农业科学—农业基础科学]