黄连素抑制高糖诱导的人视网膜血管内皮细胞凋亡  

Berberine inhibits human retinal vascular endothelial cell apoptosis induced by high glucose

作　　者：王珊珊 徐仙珍 廖星 赖美辰 付书华[1] Wang Shanshan;Xu Xianzhen;Liao Xing;Lai Meichen;Fu Shuhua(Department of Ophthalmology,The Second Affiliated Hospital of Nanchang University,Nanchang 330006,China;Department of Ophthalmology,Xiushui County Hospital of Traditional Chinese Medicine,Jiujiang 332400,China)

机构地区：[1]南昌大学第二附属医院眼科,330006 [2]江西省修水县中医院眼科,九江332400

出　　处：《中华眼底病杂志》2021年第10期790-794,共5页Chinese Journal of Ocular Fundus Diseases

基　　金：江西省卫计委中医药科技计划(2017A238)。

摘　　要：目的观察高糖环境下黄连素(BBR)对人视网膜血管内皮细胞(hREC)凋亡的抑制作用。方法将hREC分为空白对照组(NC组)、高糖组(HG组)、BBR处理组(BN组)、BBR+高糖处理组(BH组)。各组细胞均置于Dulbecco改良Eagle培养基中培养。NC组、HG组培养基中分别加入5.5、30.0 mmol/L葡萄糖。BN组培养基中加入5.0 mmol/L葡萄糖和5.0 mmol/L BBR;BH组培养基中加入30.0 mmol/L葡萄糖和5.0 mmol/L BBR。采用流式细胞仪观察各组细胞凋亡率;蛋白免疫印迹法(Western blot)检测各组细胞中B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)、细胞色素C(Cyt-C)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)蛋白相对表达量。两组间差异采用t检验,多组间差异采用单因素方差分析。结果流式细胞仪检测结果显示,与NC组比较,HG组细胞凋亡率明显升高,差异有统计学意义(P<0.01);与HG组比较,BH组细胞凋亡率明显降低,差异有统计学意义(P<0.05)。Western blot检测结果显示,与NC组比较,HG组细胞中Bax、Caspase-3蛋白相对表达量升高,Bcl-2蛋白相对表达量降低,差异均有统计学意义(P<0.01);与HG组比较,BH组细胞中Bax、Cyt-C、Caspase-3蛋白相对表达量降低,Bcl-2蛋白相对表达量升高,差异均有统计学意义(P<0.01)。结论高糖环境下BBR可通过影响凋亡蛋白表达抑制hREC凋亡。Objective To observe the inhibitory effect of berberine(BBR)on the apoptosis of human retinal vascular endothelial cells(hREC)under high glucose environment.Methods hREC was divided into blank control group(NC group),high glucose group(HG group),BBR treatment group(BN group),and BBR+high glucose treatment group(BH group).The cells of each group were cultured in Dulbecco's modified eagle medium;5.5 and 30.0 mmol/L glucose were added to the medium of the NC group and HG group,respectively;5.0 mmol/L glucose and 5.0 mmol/L BBR was added to the BN group;30.0 mmol/L glucose and 5.0 mmol/L BBR was added to the medium of the BH group.Flow cytometry was used to observe the apoptosis rate of each group.Western blotting was used to detect the relative expression levels of B-cell lymphoma-2(Bcl-2),Bcl-2 related X protein(Bax),and Cytochrome C(Cyt-C)and cysteine aspastic acid-specific protease 3(Caspase-3)proteins in each group of cells.The difference between the two groups was tested by t test,and the difference among multiple groups was analyzed by one-way analysis of variance.Results The results of flow cytometry showed that compared with the NC group,the apoptosis rate of the HG group significantly increased,and the difference was statistically significant(P<0.01);compared with the HG group,the apoptosis rate of the BH group significantly reduced,the difference was statistical significance(P<0.05).Western blot test results showed that,compared with the NC group,the relative expression of Bax and Caspase-3 protein in the HG group increased,and the relative expression of Bcl-2 protein decreased.The difference was statistically significant(P<0.01).Compared with the HG group,the relative expression of Bax,Cyt-C,and Caspase-3 protein in BH group cells decreased,and the relative expression of Bcl-2 protein increased,and the difference was statistically significant(P<0.01).Conclusion BBR can inhibit hREC apoptosis by affecting the expression of apoptotic protein under high glucose environment.

关 键 词：糖尿病视网膜病变 内皮 血管 细胞凋亡 黄连素 

分 类 号：R285[医药卫生—中药学]