增温对南亚热带针阔叶混交林凋落物分解酶活性的影响  

Effect of warming on litter decomposition enzyme activity in a southern subtropical coniferous and broad-leaf mixed forest

作　　者：程严 列志旸 刘旭军[1,2] 李旭 吴婷[1,2] 褚国伟[1] 孟泽 刘菊秀 CHENG Yan;LIE Zhiyang;LIU Xujun;LI Xu;WU Ting;CHU Guowei;MENG Ze;LIUJuxiu(CAS Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China;University of Chinese Academy of Sciences,Beijing 610041,China)

机构地区：[1]中国科学院华南植物园,中国科学院退化生态系统植被恢复与管理重点实验室,广州510650 [2]中国科学院大学,北京100049

出　　处：《应用与环境生物学报》2021年第4期923-929,共7页Chinese Journal of Applied and Environmental Biology

基　　金：广东省重点领域研发计划项目(2020B1111530004);广州市民生科技攻关项目(201903010021);国家自然科学基金项目(41977287,31961143023,41825020)资助。

摘　　要：凋落物分解酶可以催化凋落物分解并且影响其分解速率,在生态系统物质循环和能量流动过程中发挥着重要作用.采用海拔梯度改变而导致增温的方式探究南亚热带针阔叶混交林凋落物层6种分解酶活性对增温的响应特征及其影响因子.结果表明,湿季增温使酸性磷酸酶(AP)和纤维二糖酶(CBH)活性分别降低了36.08%和29.01%,干季增温使AP增加了49.90%,而β葡萄糖苷酶(BG)活性降低了21.07%,乙酰氨基葡萄糖苷酶(NAG)、多酚氧化酶(PPO)和过氧化物酶(POD)没有受到增温的影响.增温对凋落物碳(C)、氮(N)、磷(P)含量和微生物生物量碳(MBC)、微生物生物量氮(MBN)、微生物生物量磷(MBP)没有显著影响.季节变化显著影响了凋落物N含量、C:N、N:P和MBC(P<0.05),增温和干湿季交互作用显著影响凋落物C:P(P<0.05).冗余分析表明不同季节凋落物MBC和凋落物N:P的变化是影响增温背景下南亚热带针阔叶混交林凋落物分解酶活性的主导因子.综上,在气候变暖的情况下,凋落物分解酶活性的变化可能有助于缓解养分对微生物的限制,形成新的养分利用模式来应对气候变化.Litter decomposition enzymes can catalyze the decomposition of litter and affect the rate of litter decomposition,and play an important role in the material cycle and energy flow in forest ecosystems.A field warming experiment was conducted by translocating coniferous and broad-leaf mixed forest ecosystems from altitude of 300 m to 30 m in subtropical southern China.Six enzyme activities of litter,litter microbial biomass,and litter elements were then investigated.The results showed that the activities of acid phosphatase(AP)and cellobiohydrolase(CBH)were decreased by 36.08%and 29.01%,respectively,due to warming during the wet season.In the dry season,warming decreased the activity ofβ-glucosidase(BG)by 21.07%,whereas warming increased that of AP by 49.9%.The activities ofβ-1,4-N-acetylglucosaminidase(NAG),polyphenol oxidase(PPO),and peroxidase(POD)were not affected by warming.Warming had no significant effects on the C,N,P,microbial biomass C(MBC),microbial biomass N(MBN),and microbial biomass P(MBP)content of litter;however,seasonal changes significantly influenced the N,C:N,N:P,and MBC content of litter.The interactions between warming and season significantly affected the litter C:P ratio.Redundancy analysis showed that changes in litter MBC and N:P in different seasons were the main factors affecting the activities of litter decomposition enzymes in coniferous and broad-leaf mixed forest.Changes in litter enzyme activities would mitigate nutrient limitation to microbes and form new nutrient use patterns in response to climate warming.

关 键 词：增温 凋落物质量 凋落物微生物生物量 凋落物分解酶活性 干湿季节变化 南亚热带 

分 类 号：S714[农业科学—林学]