LncRNA BBOX1-AS1通过miR-185-5p/RHOA调节卵巢癌细胞放疗敏感性  

LncRNA BBOX1-AS1 regulates radiosensitivity of ovarian cancer cells via miR-185-5p/RHOA

作　　者：李慧[1] 雷垣生[2] 李双雪[3] 李峰 雷洁赟 Li Hui;Lei Yuansheng;Li Shuangxue;Li Feng;Lei Jieyun(Department of Gynaecology,Shanxi Provincial People’s Hospital,Taiyuan 030012,China;Department of Neurology,the Second Hospital of Shanxi Medical University,Taiyuan 030012,China;Respiratory Department,Shanxi Provincial People’s Hospital,Taiyuan 030012,China;Department of General Surgery,TISCO General Hospital,Taiyuan 030003,China;Gynaecology,Taiyuan Central Hospital,Taiyuan 030009,China)

机构地区：[1]山西省人民医院妇科,太原030012 [2]山西医科大学第二医院神经内科,太原030012 [3]山西省人民医院呼吸科,太原030012 [4]太钢总医院普外科,太原030003 [5]太原市中心医院妇产科,030009

出　　处：《中华内分泌外科杂志》2021年第5期499-503,共5页Chinese Journal of Endocrine Surgery

基　　金：国家自然科学基金(81101233)。

摘　　要：目的探究LncRNA BBOX1-AS1在卵巢癌(ovarian cancer,OC)放疗敏感性中的作用及潜在机制。方法qRT-PCR检测OC组织和细胞中BBOX1-AS1、miR-185-5p的表达。双荧光素酶报告实验确认BBOX1-AS1、miR-185-5p、RHOA之间的相互作用。MTT及流式细胞术观测OC细胞的增殖和凋亡。结果BBOX1-AS1在OC组织和细胞中上调,相对于si-NC组在2、3、4天的细胞增殖活力(0.89±0.07)(1.48±0.13)(1.69±0.15),si-BBOX1-AS1组的增殖活力(0.59±0.06)(0.97±0.09)(1.21±0.10)显著下降(均P<0.05)。相对于si-NC组(6.24±0.28),敲减BBOX1-AS1能诱导OC细胞的凋亡(12.07±1.33)(均P<0.05)。miR-185-5p在OC组织和细胞中表达下调,BBOX1-AS1与miR-185-5p、RHOA与miR-185-5p的靶向关系被证明。敲低miR-185-5p能逆转BBOX1-AS1对OC细胞的影响(均P<0.05)。结论LncRNA BBOX1-AS1通过调控miR-185-5p/RHOA轴抑制OC细胞放疗敏感性。Objective To investigate the potential mechanism and effects of LncRNA BBOX1-AS1 in radiosensitivity of ovarian cancer.Methods qRT-PCR was used to explore BBOX1-AS1,miR-185-5p expression in OC tissue and cells.Dual luciferase reporter assay was used to confirm the interaction of BBOX1-AS1,miR-185-5p and RHOA.MTT assay and flow cytometry were used to detect the proliferation and apoptosis of OC cells.Results BBOX1-AS1 was up-regulated in OC tissue and cells,compared with the cell proliferative activity at 2,3,4 day(0.89±0.07)(1.48±0.13)(1.69±0.15)in si-NC group,proliferative activity in si-BBOX1-AS1 group(0.59±0.06)(0.97±0.09)(1.21±0.10)was obviously down-regulated(all P<0.05).Compared with si-NC group(6.24±0.28),silencing of BBOX1-AS1 induced apoptosis(12.07±1.33)(all P<0.05).miR-185-5p was down-regulated in OC tissue and cells,the targeting relationship between BBOX1-AS1 and miR-185-5p,RHOA and miR-185-5p was confirmed.Inhibition of miR-185-5p reversed the effects of BBOX1-AS1 on OC cells(all P<0.05).Conclusion LncRNA BBOX1-AS1 inhibits radiotherapy sensitivity of OC cells via regulating miR-185-5p/RHOA axis.

关 键 词：BBOX1-AS1 miR-185-5p RHOA 卵巢癌 放疗敏感性 

分 类 号：R711.75[医药卫生—妇产科学]