HPLC测定野烟叶中的1,5-二咖啡酰奎宁酸  

Determination of 1,5-O-dicaffeoylquinic acid in Carpesii Herba by HPLC

作　　者：孙建彬[1,2] 王欣[2] 李钰婷[2] 钟国跃 吴瑞军 覃瑶 罗维早[2] SUN Jianbin;WANG Xin;LI Yuting;ZHONG Guoyue;WU Ruijun;QIN Yao;LUO Weizao(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu,Sichuan,611137 P.R.China;Chongqing Institute of Traditional Chinese Medicine,Chongqing,400065 P.R.China;Research Center far Traditional Chinese Medicine Resources and Ethnic Medicine,Jiangxi University of Traditional Chinese Medicine,Nanchang,Jiangxi,330004 P.R.China;Chongqing Taiji Industry(Group)Co.,Ltd.,Chongqing,401123 P.R.China)

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]重庆市中药研究院,重庆400065 [3]江西中医药大学中药资源与民族药研究中心,江西南昌330004 [4]重庆太极实业(集团)股份有限公司,重庆401123

出　　处：《华西药学杂志》2021年第5期554-557,共4页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用串联四极杆飞行时间质谱鉴别苗药野烟叶的指标成分,并用HPLC法测定野烟叶中指标成分1,5-二咖啡酰奎宁酸(CQA)的含量。方法采用Inertsil ODS-SP色谱柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.4%甲酸(14:86),检测波长325 nm,流速0.7 mL·min^(-1),柱温30℃。结果CQA的线性范围为0.061~3.882μg,加样回收率为93.14%,RSD=1.74%。结论所用方法简便、准确、可靠、可用于野烟叶的质量控制。OBJECTIVE To identify the index components of Carpesii Herba by QTOF-MS/MS and to determine 1,5-O-dicaffeoylquinic acid(CQA)in Herba Carpesii by HPLC.METHODS The Inertsil ODS-SP(150 mm×4.6 mm,5μm)column was used.The mobile phase was CH_(3)CN-0.4%methanolic acid solution with the flow rate of 0.7 mL·min^(-1).The detective wavelength was 325 nm and the column temperature was 30℃.RESULTS The linear range of CQA was at 0.061-3.882μg.The average recovery was 93.14%and RSD was 1.74%.CONCLUSION The method is simple,accurate and can be used for the quality control of Carpesii Herba.

关 键 词：苗药 野烟叶 串联四极杆飞行时间质谱 高效液相色谱 1 5-二咖啡酰奎宁酸 结构鉴定 含量测定 质量控制 

分 类 号：R917[医药卫生—药物分析学]