烯效唑浸种对干旱胁迫下大麻叶片的转录组调控分析  

Transcriptome Regulation Analysis of Uniconazole Seed Soaking on Hemp Leaves under Drought Stress

作　　者：姜颖[1,2] 王晓楠 张晓艳[1] 赵越 韩承伟[1] 曹焜[1] 韩喜财 边境[1] 孙宇峰 冯乃杰[2] Jiang Ying;Wang Xiaonan;Zhang Xiaoyan;Zhao Yue;Han Chengwei;Cao Kun;Han Xicai;Bian Jing;Sun Yufeng;Feng Naijie(Daqing Branch of Heilongjiang Academy of Sciences,Daqing,163319;College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing,163319)

机构地区：[1]黑龙江省科学院大庆分院,大庆163319 [2]黑龙江八一农垦大学农学院,大庆163319

出　　处：《分子植物育种》2021年第19期6376-6385,共10页Molecular Plant Breeding

基　　金：国家重点研发专项(2016YFC0501207-03);黑龙江省科学院青年创新基金(CXJQ2018DQ01);黑龙江省科学院科学研究基金(ZS2019)共同资助。

摘　　要：为了从转录组水平分析烯效唑缓解干旱胁迫的分子机制,以大麻品种‘汉麻2号’为材料,试验共设清水浸种的正常供水(CK),清水浸种的干旱处理(D),烯效唑浸种的干旱处理(SD) 3个处理,干旱胁迫处理4 d,利用RNA-Seq技术对叶片进行转录组测序分析并探讨半乳糖代谢、植物激素信号转导和氮代谢通路及相关基因。结果表明,CK vs D与D vs SD比较发现,全部差异表达基因有2 423个,其中不同处理共有差异表达基因1 109个。通过GO富集分析表明,两个比较中有1 402和1 144个差异表达基因在生物学过程、细胞组分和分子功能均有分布,且分类结果相似。差异基因GO主要富集在蛋白质折叠、氧化还原过程、胞浆、叶绿体包膜、水解酶活性、氧化还原酶活性等功能。KEGG富集结果表明,差异基因KEGG主要富集在半乳糖代谢、苯丙酸生物合成、植物激素信号转导、氮代谢、丙氨酸、天冬氨酸和谷氨酸代谢、氨基酸的生物合成等代谢途径。本研究主要分析了半乳糖代谢、植物激素信号转导和氮代谢途径,其中半乳糖代谢中UDP糖焦磷酸化酶基因,UDP葡萄糖4-差向异构酶基因,棉子糖合酶基因,水苏糖合酶基因,β-呋喃果糖苷酶基因等,植物激素信号转导中生长素响应蛋白,脱落酸受体,蛋白磷酸酶,脱落酸不敏感蛋白等,氮代谢中高亲和性硝酸盐转运蛋白,谷氨酸脱氢酶基因,谷氨酰胺合成酶基因和碳酸酐酶基因在烯效唑处理下表达水平发生变化。本研究为深入了解烯效唑处理对大麻响应干旱胁迫的分子调控机制、关键基因克隆以及功能验证等提供研究基础和理论依据。In order to analyze the molecular mechanism of uniconazole in alleviating drought stress at gene level,using hemp cultivar ’Hanma No.2’ as materials, three treatments as normal water supply of seed soaking with water(CK), drought treatment of seed soaking with water(D) and drought stress of seed soaking with uniconazole(SD)were carried out. RNA-Seq technology was used to analyze the transcriptome of leaves, then to explore the galactose metabolism, plant hormone signal transduction, nitrogen metabolism pathways and related genes after drought stress for 4 d. The results showed that there were 2 423 differentially expressed genes(DEGs) between CK vs D and D vs SD that contained 1 109 common DEGs. 1 402 and 1 144 DEGs among two comparisons were distributed in biological process, cell components and molecular functions by GO enrichment analysis. The DEGs were mainly enriched in the functions of protein folding, redox process, cytoplasm, chloroplast envelope, hydrolase activity, oxidoreductase activity and so on. KEGG enrichment results revealed that the DEGs were mainly enriched in galactose metabolism, phenylalanine biosynthesis, plant hormone signal transduction, nitrogen metabolism, alanine, aspartate and glutamic acid metabolism, amino acid biosynthesis and so on. This study mainly analyzed galactose metabolism, plant hormone signal transduction and nitrogen metabolism pathways. Among them, the expression levels of genes of UDP glucose pyrophosphorylase, UDP glucose 4-epimerase, raffinose synthase, stachyose synthase, β-fructofuranosidase in galactose metabolism, genes of auxin response protein, abscisic acid receptor, protein phosphatase, abscisic acid insensitive protein in plant hormone signal transduction, genes of high affinity nitrate transporter, glutamate dehydrogenase, glutamine synthetase and carbonic anhydrase in nitrogen metabolism were changed with uniconazole treatment. This study provides research and theoretical basis for further understanding the molecular regulatory mechanism, cloning of k

关 键 词：大麻 烯效唑 干旱胁迫 转录组 差异表达基因 

分 类 号：S563.3[农业科学—作物学]