含斑茅血缘的甘蔗回交后代遗传多样性SSR分析  

SSR Analysis of Sugarcane Backcross Progenies Contained Consanguinity of Saccharum arundinaceum Retz.

作　　者：周峰 符成 谢静 张垂明 胡后祥 王勤南 邱永生 Zhou Feng;Fu Cheng;Xie Jing;Zhang Chuiming;Hu Houxiang;Wang Qingnan;Qiu Yongsheng(Guangdong Sugarcane Germplasm Resources and Utilization of Scientific Observation Test Station,Ministry of Agriculture&Rural Affairs,Hainan Sugarcane Breeding Station,Institute of Bioengineering,Guangdong Academy of Sciences,Sanya,572025)

机构地区：[1]广东省科学院生物工程研究所,海南甘蔗育种场,农业农村部广东甘蔗种质资源与利用科学观测实验站,三亚572025

出　　处：《分子植物育种》2021年第19期6428-6437,共10页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31701488);广东省科技计划项目(2016A020210016);现代农业产业技术体系建设专项资金项目(CARS-170107);广东省甘蔗种质资源库建设项目(2019B030316034);广东省甘蔗剑麻产业技术体系创新团队专项资金项目(2019KJ104-02)共同资助。

摘　　要：利用SSR分子标记对44个含斑茅血缘的甘蔗回交后代和38个常规甘蔗栽培种进行遗传多样性分析。结果显示,在80对SSR引物中筛选出17对扩增带型清晰、多态性较好的引物,共扩增出40条多态性条带,每对引物的扩增条带数介于1~5条之间,平均2.4条。引物检测到的有效等位基因数(Ne)介于1~3.1,平均值为1.809 8;多态性信息量(PIC)介于0~0.611,平均值为0.307 9;Shanoon指数(I)介于0~1.238 2,平均值为0.591 2;Ne’s基因多样性指数(H)介于0~0.677 8,平均值为0.357 4。82份试验材料之间的遗传相似系数介于0.425~0.975之间,平均值为0.679,含斑茅血缘甘蔗回交后代品系间的平均遗传相似系数为0.700,常规甘蔗栽培种间的平均遗传相似系数为0.673,而含斑茅血缘甘蔗回交后代与常规甘蔗栽培种间的平均遗传相似系数为0.666。采用非加权组平均法(UPGMA)聚类分析表明,在遗传相似系数为0.59处,所有材料被划分为2个类群,‘台糖95-8899’独成一类,其他81个材料划分为另外一个类群,定为A类群;在遗传相似系数0.612处,A类群被划分为2个亚类群,‘崖城03-191’独成一类,其余80个材料划分为另外一个亚类群。本研究基于SSR分子标记技术,揭示了含斑茅血缘的甘蔗回交后代的遗传多样性,为含斑茅血缘的甘蔗回交后代的杂交利用提供了一定的参考依据。The genetic diversity of forty-foursugarcane backcross progeniescontained consanguinity of Saccharum arundinaceum Retz. and thirty-eightconventional sugarcane cultivars was analyzed by SSR molecular markers. The result showed that among the eight SSR primers, seventeenpolymorphic primers which can amplify clear bands were screened out and these picked out primers amplified forty distinct polymorphic bands, the number of amplified bands of every primer was between 1 to 5, average number was 2.4. The effective number of allele(Ne)was between 1 to 3.1, and the mean value was 1.809 8;the polymorphism information content(PIC) was between0~0.611, and the mean value was 0.307 9. The shannon’s information index(I) was between 0 to 1.238 2, and the mean value was 0.591 2. The nei’s gene diversity(H) was between 0~0.677 8, and the mean value was 0.357 4. The mean similaritycoefficientof eighty-two experiment materials was between 0.425 to 0.975, and the average value was 0.679, the mean similarity coefficient between different sugarcane backcross prognies contained consanguinity of Saccharum arundinaceum Retz. was 0.700, and the mean similarity coefficient between different conventional sugarcane cultivars was 0.673, and the mean similarity coefficient between sugarcane backcross progny contained consanguinity of Saccharum arundinaceum Retz. and conventional sugarcane cultivar was 0.666. The cluster analysis by the method unweighted pair group method of arithmetic average(UPGMA) illustrated that when the value was 0.59 on the similarity coefficient scale, all the experiment materials were divided into two groups,’TT95-8899’ was the only one in the therein group, and the other eighty-one experiment materials were in another group which named group A;When the value was 0.612 on the similarity coefficient scale, the group A was divided into two subgroups, ’YC03-191’ was the only one in the therein group, and the other eighty experiment materials were in another subgroup. In this study, SSR molecular marker stechno

关 键 词：甘蔗 斑茅(Saccharum arundinaceum Retz.) 遗传多样性 SSR 

分 类 号：S566.1[农业科学—作物学]