低温胁迫下黄花苜蓿根部的渗透调节和抗氧化能力变化  

Changes of osmotic adjustment and antioxidant capacity of roots of Medicago falcata L. under low temperature stress

作　　者：张攀 柴华 杨梅 尹航 张茜 金福恒 崔国文[1] ZHANG Pan;CHAI Hua;YANG Mei;YIN Hang;ZHANG Qian;JIN Fuheng;CUI Guowen(College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China;Animal Husbandry and Veterinary Branch,Heilongjiang Academy of Agricultural Sciences,Qiqihar 161005,China;Forestry and Grassland Administration of Hinggan League,Hinggan League 137400,China;Hinggan League State-owned Forest Farm and Forest Park Administration,Hinggan League 137400,China)

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔161005 [3]兴安盟林业和草原局,内蒙古兴安盟137400 [4]兴安盟国有林场和森林公园管理局,内蒙古兴安盟137400

出　　处：《黑龙江畜牧兽医》2021年第19期113-117,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江省普通本科高等学校青年创新人才培养计划项目(UNPYSCT-2017020)。

摘　　要：为了探明低温胁迫对黄花苜蓿根部渗透调节和抗氧化能力的影响,试验以呼伦贝尔黄花苜蓿(Medicago falcata cv.Hulunbeier)种子为试验材料,分析了不同低温(4,0,-5,-10,-15℃)和不同时间(0,4,8,12 h)处理下黄花苜蓿根部电解质外渗率、脯氨酸含量、可溶性糖含量、还原型谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性等生理指标的变化。每个处理进行4个重复,每个重复4株黄花苜蓿植株。结果表明:低温胁迫能够造成黄花苜蓿根部细胞损伤,导致电解质外渗率增加。低温胁迫使黄花苜蓿根部脯氨酸和可溶性糖含量增加,且随胁迫温度降低二者含量增加,以维持黄花苜蓿根部细胞渗透压;不同胁迫时间对黄花苜蓿根部抗氧化能力影响明显,随胁迫时间延长,根部SOD、POD和CAT活性波动上升;不同胁迫温度下黄花苜蓿根部GSH含量均为低温胁迫4 h最高。说明在低温胁迫过程中黄花苜蓿根部抗氧化能力逐步增强,进而抵御活性氧对黄花苜蓿造成的伤害。In order to explore the effect of low temperature stress on osmotic adjustment and antioxidant capacity of the roots of Medicago falcata L.(M.falcata),seeds of M.falcata cv. Hulunbeier was selected as the test materials. Physiological indicators in the roots of M.falcata, including electrolyte extravasation rate, proline content, soluble sugar content, reduced glutathione(GSH) content and the activities of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) were analyzed under different low temperatures(4, 0,-5,-10,-15 ℃) and different times(0, 4, 8, 12 h).Every treatment with 4 replicates per treatment, and 4 plants were selected for each replicate. The result showed that low temperature stress could damage the root cells of M.falcata, resulting in an increase in its electrolyte extravasation rate.The content of proline and soluble sugar in the roots of M.falcata increased under low temperature stress, and the content of both increased with the decrease of stress temperature, so as to maintain the osmotic pressure of the roots’ cells. The stress time played a significant effect on the antioxidant capacity of M.falcata. The activities of SOD, POD and CAT increased wavily with the elongation of stress time, and the content of GSH at 4 h was the highest under different temperature point. The results indicated that the antioxidant capacity of the roots of M.falcata gradually enhanced during the process of low temperature stress, and thus it could resist the damage caused by active oxygen to the M. falcata.

关 键 词：黄花苜蓿 低温胁迫 细胞膜损伤 渗透调节 活性氧 

分 类 号：S551.7[农业科学—作物学]