比索洛尔对心力衰竭兔心肌细胞L型钙通道的影响  

Effect of bisoprolol on I in volume overload combined with pressure overload heart failure in rabbits

作　　者：李溪 王亭忠[2] 卢群[2] 韩克[2] 卓小桢 马爱群[2] LI Xi;WANG Tingzhong;LU Qun;HAN Ke;ZHUO Xiaozhen;MA Aiqun(Department of Cardiology,General hospital of Ningxia Medical University,Yinchuan 750001;Department of Internal Medicine,The First Afflicted Hospital of Xi’an Jiaotong University,Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education/Key Laboratory of Medical School of Molecular Cardiology,Xi’an 710061,China)

机构地区：[1]宁夏医科大学总医院心脑血管病医院心内科,宁夏银川750001 [2]西安交通大学第一附属医院心内科,教育部环境与疾病相关基因重点实验室,陕西省分子心脏病学重点实验室,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2021年第6期823-828,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金重点资助项目(No.30830051)。

摘　　要：目的探讨比索洛尔对心力衰竭兔心肌细胞ICa,L通道的影响。方法选择82只新西兰兔制备容量联合压力超负荷心力衰竭兔模型,随机分为假手术组(SO)、心力衰竭组(HF)、比索洛尔干预组(BF)。通过Real-time PCR和Western blotting法检测比索洛尔对心衰兔左室心肌细胞ICa,L表达的影响;通过膜片钳技术检测比索洛尔对心衰兔左室心肌细胞ICa,L功能的影响。结果 (1)心脏彩超及BNP等结果显示比索洛尔可以改善HF兔的心脏重构(P<0.05);(2)HF组兔心肌细胞ICa,L通道mRNA和蛋白的表达明显减低,BF组ICa,L通道表达没有上调(P>0.05);(3)HF兔心肌细胞电容增大,ICa,L激活速度加快,电流密度明显降低;BF组心肌细胞电容、ICa,L激活和电流密度没有明显改变;(4)HF组ICa,L通道激活曲线左移,使窗电流(window current)增大,BF组ICa,L通道的失活速度加快,使窗电流减小。结论比索洛尔可以改善心衰兔心功能,还可以影响心衰兔心肌细胞ICa,L的功能。Objective To explore the effect of bisoprolol on ICa,L in volume overload combined with pressure overload heart failure in rabbits. Methods Totally 82 male New Zealand rabbits(2. 5-3. 0 kg)were randomly divided into three groups:SO(sham group), HF(heart failure group), and BF(heart failure with bisoprolol treatment group).HF rabbits were duplicated by aortic valve insufficiency procedure combined with abdominal aorta constriction procedure.Real-time PCR and Western blot analysis were performed to detect the expression of ICa,L in left ventricular myocytes. Left ventricular myocytes were isolated;then the cell membrane capacitance,the current density,activation and inactivation of ICa,L were recorded by whole cell patch clamp. Results(1) Bisoprolol could improve the heart function of heart failure rabbits according to the measurement of echocardiography and BNP.(2) The expressions of ICa,L mRNA and protein decreased significantly in heart failing rabbits(P<0. 01), but remained unaltered after chronic bisoprolol treatment(P>0. 05).(3) Membrane capacitance was larger in heart failing groups than in sham group(P<0. 01). ICa,L current density decreased greatly in HF group(P<0. 01). Bisoprolol treatment could not change Cm or ICa,L density(P>0. 05). V1/2 of activation curve negative shift enlarged window currents in heart failure groups. Bisoprolol treatment caused window currents to decrease. Conclusion Bisoprolol could reverse the heart function of heart failure rabbits and also affect the function of ICa,L .

关 键 词：心力衰竭 离子通道 L型钙通道 比索洛尔 

分 类 号：R332[医药卫生—人体生理学]