鼠李糖乳杆菌对小鼠产后抑郁样行为和单胺神经递质及肠道菌群的影响  

Effects of Lactobacillus rhamnosus on postpartum depression-like behavior,monoamine neurotransmitter and intestinal flora in mice

作　　者：李腾[1] 周小兰[1] 褚静[1] 王立芹[1] 曹国芬[1] 张海苗[1] LI Teng;ZHOU Xiaolan;CHU Jing;WANG Liqin;CAO Guofen;ZHANG Haimiao(School of Nursing,Xi’an Medical College,Xi’an 710021,China)

机构地区：[1]西安医学院护理学院,陕西西安710021

出　　处：《西安交通大学学报（医学版）》2021年第6期862-867,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的初步探讨鼠李糖乳杆菌的产后抗抑郁作用及其潜在机制。方法在雌性小鼠妊娠期间施用地塞米松磷酸钠建立产后抑郁症(PPD)模型。适应性喂养结束后,将受孕的50只雌性小鼠随机分为鼠李糖乳杆菌干预低剂量组(Ⅰ组)、鼠李糖乳杆菌干预高剂量组(Ⅱ组)、阳性对照组(Ⅲ组)、模型对照组(Ⅳ组)和空白对照组(Ⅴ组)。通过灌胃给药进行干预,Ⅰ组和Ⅱ组小鼠分别给予鼠李糖乳杆菌1×107和1×108CFU(kg·d),Ⅲ组小鼠给予1.8 mg/(kg·d)帕罗西汀,Ⅳ组和Ⅴ组小鼠给予等量的生理盐水,干预时间为4周。通过24 h食物消耗实验、旷场实验、糖水消耗实验检测各组小鼠的行为学表现,反相高效液相色谱法测定5-羟色胺(5-HT)、去甲肾上腺素(NE)及多巴胺(DA)的浓度,RT-qPCR法检测小鼠盲肠中粪肠球菌、双歧杆菌、乳酸杆菌和大肠杆菌的变化。结果造模前各组小鼠摄食量、体质量变化率、旷场移动距离和速度、糖水消耗百分比差异均无统计学意义(P>0.05)。造模后与Ⅴ组相比,Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组小鼠的摄食量和体质量变化率差异无统计学意义,而旷场移动距离、移动速度和糖水偏爱百分比明显降低,差异有统计学意义(P<0.05)。鼠李糖乳杆菌干预后,与Ⅳ组相比,Ⅰ组、Ⅱ组小鼠的抑郁样行为得到改善,体质量变化率、旷场移动距离和速度、糖水偏爱百分比和单胺神经递质浓度较Ⅳ组显著增加(P<0.05),粪肠球菌、大肠杆菌和乳酸杆菌显著下降(P<0.05),双歧杆菌有上升趋势,但未见统计学差异;摄食量没有明显变化。结论鼠李糖乳杆菌能够改善产后小鼠抑郁样行为,影响小鼠单胺类神经递质,调节肠道菌群,为产后抑郁相关研究提供了新的方向。Objective To explore the effect of Lactobacillus rhamnosus on postpartum depression(PPD)and its potential mechanism. Methods The mouse model of PPD was established by using dexamethasone sodium phosphate during pregnancy. At the end of adaptive feeding, 50 pregnant female mice were randomly divided into low-dose group(group Ⅰ), high-dose group(Group Ⅱ), positive control group(Group Ⅲ), model control group(Group Ⅳ), and blank control group(Group Ⅴ). The mice in Group Ⅰ and Group Ⅱ were given Lactobacillus rhamnosus 1×107 and 1×108 CFU(kg·d). The mice in Group Ⅲ were given 1. 8 mg/(kg·d)paroxetine,and the mice in Groups Ⅳ and Ⅴ were given the same amount of normal saline for 4 weeks. The 24-hour food consumption test,open field test and sugar water consumption test were used to detect the behavior of mice in each group. The concentrations of 5-hydroxytryptamine(5-HT), norepinephrine(NE), and dopamine(DA) were determined by RP-HPLC. The changes in Enterococcus faecalis,Bifidobacterium,Lactobacillus and Escherichia coli in the cecum of mice were detected by Real-time fluorescence quantitative PCR(RT-qPCR). Results Before modeling, there were no significant differences in food intake, weight change rate, open field moving distance and speed, and percentage of sugar consumption among the groups(P>0. 05).After modeling, there was no significant difference in food intake or weight change rate among the five groups, but the open field moving distance, moving speed and percentage of sugar preference were significantly reduced(P<0. 05).After intervention with Lactobacillus rhamnosus, compared with Group Ⅳ, the depression-like behavior in Group Ⅰ and Group Ⅱ mice was improved;the weight change rate, open field moving distance and speed, percentage of sugar preference,and monoamine neurotransmitter concentration in Group Ⅰ and Group Ⅱ were significantly increased compared with those in Group Ⅳ (P<0. 05), while Enterococcus faecalis, Escherichia coli and lactobacillus were significantly decr

关 键 词：鼠李糖乳杆菌 产后抑郁 行为学表现 单胺类神经递质 

分 类 号：R378[医药卫生—病原生物学] R922[医药卫生—基础医学]