基于信息熵理论的地桃花叶醇提工艺优选及其化学成分的研究  

Study on Optimization of Ethanol Extraction Technology and Chemical Constituents from the Leaves of Urena lobata Linn.based on Information Entropy Theory

作　　者：韦正 张鹏 宁东元 宁廷婷 鲁世明 张晖英 WEI Zheng;ZHANGPeng;NIING Dongyuan;NING Tingting;LU Shiming;ZHANG Huiying(School of Chemistry and Biological Engeering,Hechi University,Guangxi Hechi 546300;School of Chemistry and Environmental Engineering,Hanshan Normal University,Guangdong Chaozhou 521041)

机构地区：[1]河池学院化学与生物工程学院,广西河池546300 [2]韩山师范学院化学与环境工程学院,广东潮州521041

出　　处：《生物化工》2021年第5期12-16,共5页Biological Chemical Engineering

摘　　要：目的:多指标综合加权评分法优选地桃花乙醇提取的最佳工艺。方法:采用正交试验法,研究乙醇浓度、提取时间、提取温度、料液比对地桃花叶乙醇提取的影响;应用紫外分光光度计法测定地桃花总黄酮含量和自由基清除率,利用高效液相色谱测定地桃花叶中银椴苷的含量,并以地桃花叶中银椴苷含量、总黄酮得率和自由基清除率为参考指标,采用加权评分法进行综合评判。结果:确定最佳的提取温度为95℃,乙醇浓度75%,提取时间120 min,料液比1∶60(g∶mL),银椴苷的含量为0.11837 mg/g,总黄酮的含量为25.22430 mg/g。结论:该方法稳定可行、重复性良好。Objective:The optimal extraction process parameters are determined by using the multi-index synthetic weighted scoring method.Methods:The influence of ethanol concentration,extraction time,extraction temperature,and liquid ratio of peachylitol is investigated by orthogonal test.Application of ultraviolet spectrophotometer method to the peach blossom flavonoids and antioxidant activity,using high-performance liquid chromatography(HPLC)-photoelectric diode array detection to the Urena lobata Linn.of the lime content of tiliroside.The indexes are evaluated by using the weighted scoring method.Results:Determine the optimal alcohol extraction process of 75%ethanol,extracting temperature is 95℃,extracting 120 min,material liquid than 1∶60(g∶mL),the content of tiliroside is 0.11837 mg/g,and the content of total flavonoids is 25.22430 mg/g.Conclusion:The method is simple,easy and repeatable.

关 键 词：地桃花 总黄酮 多指标综合加权评分法 高效液相色谱(HPLC) 信息熵 

分 类 号：R284.2[医药卫生—中药学]