软叶针葵花粉和鱼尾葵花粉致敏患者嗜碱性粒细胞活化情况研究  

Study on the basophil activation situation of patients being allergic to pollen of phoenix roebelenii and fishtail palm

作　　者：谢伟伟 林秀联 龙绮[1] 蔡琼香 XIE Weiwei;LIN Xiulian;LONG Qi;CAI Qiongxiang(Department of Otorhinolaryngology-Head and Neck Surgery,Haikou Hospital Affiliated to Xiangya School of Medicine of Central South University,Haikou,Hainan,570208)

机构地区：[1]中南大学湘雅医学院附属海口医院耳鼻咽喉头颈外科,海南海口570208

出　　处：《实用临床医药杂志》2021年第16期9-12,共4页Journal of Clinical Medicine in Practice

基　　金：国家自然科学基金资助项目(81160129);海南省自然科学基金资助项目(811145);海南省海口市重点科技计划项目(海科工信立[2012]72号)。

摘　　要：目的探讨软叶针葵花粉和鱼尾葵花粉致敏患者的嗜碱性粒细胞活化情况。方法选取83例软叶针葵花粉和鱼尾葵花粉致敏患者纳入观察组,另选取同时期83例健康体检者纳入对照组。比较2组嗜碱性粒细胞活化指标(CD63、CD203c及CD45)荧光强度,并比较观察组内不同过敏原类型、过敏原种数及UniCAP分级者的检测结果,采用Spearman秩相关分析探讨嗜碱性粒细胞活化指标荧光强度与UniCAP分级的关系。结果观察组的嗜碱性粒细胞CD63、CD203c及CD45的荧光强度为(32.26±2.23)、(50.31±5.61)及(36.36±5.03),分别高于对照组的(6.15±1.20)、(18.28±2.63)及(7.02±1.36),差异有统计学意义(P<0.05)。观察组内不同过敏原类型及不同过敏原种数患者的检测结果比较,差异无统计学意义(P>0.05);UniCAP分级5~6级者的嗜碱性粒细胞CD63、CD203c、CD45的荧光强度为(38.16±3.15)、(66.73±6.96)、(50.86±6.59),分别高于UniCAP分级3~4级者的(29.37±2.31)、(51.13±5.78)、(39.10±5.13)和UniCAP分级1~2级者的(18.68±1.76)、(29.36±3.79)、(22.32±1.93),且UniCAP分级3~4级者高于UniCAP分级1~2级者,差异均有统计学意义(P<0.05);Spearman秩相关分析显示,嗜碱性粒细胞活化指标CD63、CD203c、CD45均与UniCAP分级呈正相关(P<0.05)。结论软叶针葵花粉和鱼尾葵花粉致敏患者嗜碱性粒细胞活化情况突出,且活化指标CD63、CD203c、CD45表达程度与UniCAP分级密切相关,具有较高的检测价值。Objective To investigate the basophil activation situation of patients being allergic to pollen of phoenix roebelenii and fishtail palm.Methods A total of 83 patients being allergic to pollen of phoenix roebelenii and fishtail palm were chosen as observation group,83 healthy persons at the same time were chosen as control group.The fluorescence intensity of basophil activation situation(CD63,CD203c and CD45)of two groups were compared,and the detection results of observation group with different allergens,kinds and UniCAP classifications were compared,and the relationships between the fluorescence intensity of basophil activation indexes and UniCAP classifications were analyzed by Spearman rank correlation analysis.Results The fluorescence intensities of basophil activation of CD63,CD203c and CD45 of the observation group were(32.26±2.23),(50.31±5.61)and(36.36±5.03),respectively,and they were significantly higher than(6.15±1.20),(18.28±2.63)and(7.02±1.36)of the control group(P<0.05).There were no statistically significant differences in detection results of the observation group with different allergens and kinds(P>0.05).The fluorescence intensities of basophil activation CD63,CD203c and CD45 of the observation group with UniCAP classifications 5 to 6 were(38.16±3.15),(66.73±6.96)and(50.86±6.59),respectively,which were significantly higher than(29.37±2.31),(51.13±5.78)and(39.10±5.13)of UniCAP classifications 3 to 4 and(18.68±1.76),(29.36±3.79)and(22.32±1.93)of 1 to 2 grading of UniCAP classification.The Spearman rank correlation analysis showed the basophil activation indexes such as CD63,CD203c and CD45 were positively correlated with UniCAP classifications(P<0.05).Conclusion The basophil activation situation of patients being allergic to pollen of phoenix roebelenii and fishtail palm are obvious,and the expression degree are close to the UniCAP classifications,so the detection value are higher.

关 键 词：软叶针葵花粉 鱼尾葵花粉 变态反应 嗜碱性粒细胞活化 UniCAP分级 

分 类 号：R392.8[医药卫生—免疫学] R76[医药卫生—基础医学]