Cdc42及YAP检测对非瓣膜性心房颤动诊断的相关性研究  

Correlation between Cdc42 and YAP in the diagnosis of nonvalvular atrial fibrillation

作　　者：唐碧[1] 徐庆梅 朱飞宇 莫辰 张恒[1] 宣玲[1] 汤阳[1] 王闯 康品方[1] TANG Bi;XU Qing-mei;ZHU Fei-yu;MO Chen;ZHANG Heng;XUAN Ling;TANG Yang;WANG Chuang;KANG Pin-fang(Department of Cardiovascular,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004;Research Center of Cardiovascular and Cerebrovascular Diseases,Bengbu Medical College,Bengbu Anhui 233030,China;School of Clinical Medicine,Bengbu Medical College,Bengbu Anhui 233030,China)

机构地区：[1]蚌埠医学院第一附属医院心内科,安徽蚌埠233004 [2]蚌埠医学院心脑血管病研究中心,安徽蚌埠233030 [3]蚌埠医学院临床医学院,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2021年第10期1349-1353,共5页Journal of Bengbu Medical College

基　　金：教育部留学回归人员科研启动基金(第46批-教外司留〔2013〕693号);国家自然科学基金面上项目(81170803);国家自然科学基金资助项目(81970313);安徽省自然科学基金青年基金资助项目(1908085QH353);蚌埠医学院512人才培育计划项目(by51201214);安徽省教育厅重大项目(KJ2018ZD023)。

摘　　要：目的:探讨非瓣膜性心房颤动(房颤)病人血清细胞分裂周期蛋白42(Cdc42)和转录辅助激活因子Yes相关蛋白(YAP)表达水平及临床意义。方法:选取81例非瓣膜性房颤病人(其中阵发性房颤38例,持续性房颤43例)为研究对象,40例同期住院非房颤病人为对照组,采用酶联免疫吸附法检测血清Cdc42及YAP表达水平。同时结合相关血清学指标,统计分析非瓣膜性房颤病人的Cdc42及YAP水平,探讨两者与非瓣膜性房颤诊断的关系。结果:同对照组相比,房颤病人CRP、国家标准化比值、D-二聚体、LAD水平增高,LVEF水平降低,差异有统计学意义(P<0.01),房颤病人中,持续性房颤病人较阵发性房颤病人LAD增大,LVEF降低,差异有统计学意义(P<0.01);同对照组相比,房颤病人血清Cdc42和YAP表达水平增高,差异有统计学意义(P<0.01),持续性房颤病人血清Cdc42和YAP表达水平较阵发性房颤病人升高,差异有统计学意义(P<0.01和P<0.05)。Pearson相关性分析结果显示,房颤病人血清Cdc42和YAP水平与D-二聚体、LAD呈正相关(P<0.05~P<0.01),与左心室射血分数呈负相关(P<0.01);Cdc42及YAP二者联合诊断房颤的受试者工作特征曲线下面积较单独诊断提高(P<0.05)。D-二聚体、LAD、Cdc42及YAP表达增加、LVEF减小均为影响房颤的危险因素(P<0.05~P<0.01)。结论:房颤病人血清Cdc42及YAP异常表达,可能参与房颤心房结构重构的发生发展,可用作早期潜在辅助诊断房颤的指标。Objective:To investigate the serum expression levels of cell division cyclin 42(Cdc42)and transcription-assisted activator Yes-associated protein(YAP)in patients with non-valvular atrial fibrillation,and its clinical significance.Methods:Eighty-one patients with non-valvular atrial fibrillation(including 38 patients with paroxysmal atrial fibrillation and 43 patients with persistent atrial fibrillation)were selected,and 40 patients with non-atrial fibrillation at the same time were set as the control group.The serum levels of Cdc42 and YAP of patients with non-valvular atrial fibrillation were detected using ELISA,and statistically analyzed in combination with related serological indexes.The relationship between the levels of Cdc42 and YAP and diagnosis of non-valvular atrial fibrillation were investigated.Results:Compared with the control group,the levels of CRP,national standardized ratio,D-dimer and LAD increased,the LVEF level decreased,and the differences of which were statistically significant(P<0.01).Compared with patients with paroxysmal atrial fibrillation,the level of LAD increased,the level of left ventricular ejection fraction(LVEF)decreased in patients with persistent atrial fibrillation,and the differences of which were statistically significant(P<0.01).Compared with the control group,the serum levels of Cdc42 and YAP increased in patients with atrial fibrillation,and the differences of which were statistically significant(P<0.01).Compared with patients with paroxysmal atrial fibrillation,the serum levels of Cdc42 and YAP increased in patients with persistent atrial fibrillation,and the differences of which were statistically significant(P<0.01).The results of Pearson correlation analysis showed that the serum levels of Cdc42 and YAP were positively correlated with the levels of D-dimer and LAD in patients with atrial fibrillation(P<0.05 to P<0.01),and negatively correlated with LVEF(P<0.01).The area under ROC curve of the combined diagnosis of atrial fibrillation with Cdc42 and YAP was significantl

关 键 词：心房颤动 心房纤维化 细胞分裂周期蛋白42 Yes相关蛋白 

分 类 号：R541.75[医药卫生—心血管疾病]