氯化钠贮存大鼠皮肤抗原性的表达及活力研究  

The antigenicity and viability of rat skin stored in sodium chloride

作　　者：刘军[1] 王涛涛 侯明奎 Jun Liu;Tao-tao Wang;Ming-kui Hou(Department of Burns,Gansu Provincial People's Hospital,Lanzhou,Gansu 730000,China)

机构地区：[1]甘肃省人民医院烧伤科,甘肃兰州730000

出　　处：《中国现代医学杂志》2021年第20期48-52,共5页China Journal of Modern Medicine

摘　　要：目的观察经液氮贮存、氯化钠处理的新鲜大鼠皮肤抗原性的表达及组织活力。方法选取SD大鼠30只,随机分为3组,每组10只;制备大鼠全层皮肤,分别应用液氮、氯化钠进行贮存,于3个月时与新鲜大鼠皮肤分别通过免疫组织化学染色、SYTO/EB活力测定法进行MHC-DR、CD86抗原表达及活力检测。结果新鲜皮肤组MHC-DR、CD86抗原表达水平较液氮冷冻组、氯化钠组高(P<0.05),液氮冷冻组与氯化钠组的MHC-DR、CD86抗原表达水平比较,差异无统计学意义(P>0.05);液氮冷冻组与氯化钠组皮肤组织活力均超过半数,但两者之间比较,差异无统计学意义(P>0.05);新鲜皮肤组皮肤组织活力较液氮冷冻组、氯化钠组高(P<0.05)。结论液氮及氯化钠处理的大鼠皮肤均可减弱皮片的抗原性,氯化钠和液氮贮存大鼠皮肤的组织活力相近。Objective To observe the antigenicity and viability of rat skin after being stored in liquid nitrogen or sodium chloride.Methods Thirty SD rats were randomly divided into 3 groups with 10 rats in each group.The full-thickness rat skin was prepared and stored in liquid nitrogen or sodium chloride.After 3 months,MHC-DR and CD86 expression and viability of the treated and fresh rat skins were detected via immunohistochemistry and SYTO/EB dyes,respectively.Results The fresh skin group exhibited the highest expression levels of MHC-DR and CD86 compared with the liquid nitrogen group and sodium chloride group(P<0.05),yet the expressions of MHC-DR and CD86 were similar in the liquid nitrogen group and sodium chloride group(P>0.05).More than 50%of the tissues were viable in both liquid nitrogen group and sodium chloride group,and the viability was comparable between the two groups(P>0.05).In contrast,the tissue viability in the fresh skin group was the highest compared with the liquid nitrogen group and sodium chloride group(P<0.05).Conclusions Treatment with liquid nitrogen and sodium chloride can weaken the antigenicity of the rat skin.Besides,the tissue viability is similar after being treated with sodium chloride or liquid nitrogen.

关 键 词：皮肤抗原 MHC-DR CD86 组织活力 大鼠 

分 类 号：R392.1[医药卫生—免疫学]