匹诺塞林对缺糖缺氧/复糖复氧损伤SH-SY5Y细胞内钙离子及Caspase-12表达的影响  

Effects of pinocembrin on intracellular calcium ion and the expression of Caspase-12 in SH-SY5Y cells injured by oxygen-glucose deprivation/reperfusion

作　　者：武彩霞 马晓茜 唐敏芳 于宗琴 李娅宁 霍连广 WU Cai-xia;MA Xiao-qian;TANG Min-fang(Shandong Medical College,Linyi 276002,China)

机构地区：[1]山东医学高等专科学校,276000

出　　处：《中国现代药物应用》2021年第19期234-237,共4页Chinese Journal of Modern Drug Application

基　　金：山东省医药卫生科技发展计划项目“匹诺塞林对内质网应激损伤SH-SY5Y细胞的保护作用及机制研究”(项目编号:2016WS0566)。

摘　　要：目的探讨匹诺塞林对缺糖缺氧/复糖复氧(OGD/R)损伤SH-SY5Y细胞的细胞内钙离子([Ca^(2+)]i)变化及Caspase-12表达的影响。方法SH-SY5Y细胞制备OGD/R损伤模型,缺糖缺氧2 h,复糖复氧12 h,荧光探针法检测细胞胞质内钙信号变化,免疫荧光检测细胞内Caspase-12表达。结果OGD/R损伤可使SH-SY5Y[Ca^(2+)]i升高,Caspase-12表达增加,匹诺塞林能降低OGD/R损伤的SH-SY5Y细胞的[Ca^(2+)]i,减少Caspase-12的表达。结论匹诺塞林能减轻OGD/R诱导的SH-SY5Y细胞损伤,作用机制可能与其降低[Ca^(2+)]i,减少Caspase-12表达有关。Objective To discuss the effects of pinocembrin on intracellular calcium ion([Ca^(2+)]i)and the expression of Caspase-12 in SH-SY5Y cells injured by oxygen-glucose deprivation/reperfusion(OGD/R).Methods SH-SY5Y cells were used to establish OGD/R injury model with oxygen-glucose deprivation for 2 h and reperfusion for 12 h,then the intracellular calcium signal was detected by fluorescent probe,and the expression of intracellular Caspase-12 was detected by immunofluorescence.Results OGD/R injury can increase SH-SY5Y[Ca^(2+)]i and increase the expression of Caspase-12.Pinocerin can reduce[Ca^(2+)]i and reduce the expression of Caspase-12 in SH-SY5Y cells injured by OGD/R.Conclusion Pinocerin can relieve the injury of SH-SY5Y cells induced by OGD/R,and the mechanism of action may be related to the reduction of[Ca^(2+)]i and the expression of Caspase-12.

关 键 词：匹诺塞林 SH-SY5Y细胞 缺糖缺氧/复糖复氧 细胞内钙离子 CASPASE-12 

分 类 号：R965[医药卫生—药理学]