低剂量ALA-PDT上调miR-23a减轻UVB诱导的成纤维细胞凋亡  

Low-dose ALA-PDT reduces UVB-induced apoptosis of fibroblasts by up-regulating miR-23a

作　　者：施智男 程佳伟 陶金成 张琳[1] 谷丽 周舒 顾丽群 花卉 SHI Zhinan;CHENG Jiawei;TAO Jincheng;ZHANG Lin;GU Li;ZHOU Shu;GU Liqun;HUA Hui(Department of Dermatology,Nantong Third Hospital Affiliated to Nantong University,Nantong 226006;Nantong University Medical School;Department of Dermatology,Nantong First People’s Hospital,Jiangsu Province)

机构地区：[1]南通大学附属南通第三医院皮肤性病科,南通226006 [2]南通大学医学院 [3]江苏省南通市第一人民医院皮肤性病科

出　　处：《南通大学学报（医学版）》2021年第4期314-318,共5页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(81903246);南通市科技计划项目(JC2019024,JC2020074);南通市卫生健康委员会科研课题(QB2020002,QA2020028)。

摘　　要：目的:探究低剂量5-氨基酮戊酸光动力疗法(5-aminolevulinic photodynamic therapy,ALA-PDT)预处理人成纤维细胞调控miR-23a减少中波紫外线(ultraviolet B,UVB)致细胞凋亡的机制。方法:将细胞分为3组:对照组、UVB组、ALA-PDT+UVB组。使用低剂量的ALA-PDT预先处理细胞,然后予60 mJ/cm^(2) UVB一次性照射细胞。采用实时定量聚合酶链式反应(real time quantity polymerase chain reaction,qRT-PCR)方法检测miR-23a的表达水平,运用细胞转染法敲低miR-23a,采用流式细胞术检测各组细胞的凋亡情况。结果:ALA-PDT+UVB组的miR-23a表达水平较UVB组显著提高,敲低miR-23a表达可降低ALA-PDT对UVB致细胞凋亡的抑制作用。结论:低剂量ALA-PDT预处理通过上调miR-23a表达明显抑制UVB诱导的细胞凋亡,减轻光损伤。Objective:To explore the mechanism by which low-dose 5-aminolevulinic photodynamic therapy(ALA-PDT)pretreatment of human fibroblasts regulates miR-23a to reduce ultraviolet B(UVB)-induced apoptosis.Methods:The cells were divided into three groups:control group,UVB group,ALA-PDT+UVB group.Use low-dose ALA-PDT to pre-treat the cells,and then irradiate the cells with 60 mJ/cm^(2) UVB once.The real time quantity polymerase chain reaction(qRT-PCR)experimental method was used to detect the expression level of miR-23a.The cell transfection method was used to knock down miR-23a,and the apoptosis of each group was detected by flow cytometry.Results:The expression of miR-23a in the ALA-PDT+UVB group was significantly higher than that in the UVB group.Knockdown of miR-23a can reduce the inhibitory effect of ALA-PDT on UVB-induced apoptosis.Conclusion:Low-dose ALA-PDT pretreatment can significantly inhibit UVB-induced cell apoptosis and reduce light damage by enhancing miR-23a expression.

关 键 词：低剂量5-氨基酮戊酸光动力疗法 miR-23a 中波紫外线 人成纤维细胞 急性光损伤 

分 类 号：R751[医药卫生—皮肤病学与性病学]