活血灵方对大鼠下肢深静脉血栓形成及Notch2/Hes-1表达的影响  

Effect of Huoxueling Formula on DVT of Lower Limbs and Notch2/Hes-1 Expression in Rats

作　　者：侯彦杰[1] 李小玲[1] 刘园园 马亮英[1,2] HOU Yanjie;LI Xiaoling;LIU Yuanyuan;MA Liangying(The Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830063,China;The Seventh Affiliated Hospital of Xinjiang Medical University,Urumqi 830028,China)

机构地区：[1]新疆医科大学第二附属医院,新疆乌鲁木齐830063 [2]新疆医科大学第七附属医院,新疆乌鲁木齐830028

出　　处：《中医药信息》2021年第10期23-28,共6页Information on Traditional Chinese Medicine

基　　金：新疆维吾尔自治区自然科学基金项目(2019D01C236)。

摘　　要：目的:探讨活血灵方对大鼠下肢深静脉血栓形成(Deepvenousthrombosis,DVT)及Notch2/Hes-1通路的影响。方法:50只SD大鼠随机分为对照组、模型组和活血灵方低剂量组(6.3 g/kg)、中剂量组(12.6 g/kg)、高剂量组(25.2 g/kg),每组10只。对照组仅进行缝合伤口,其余各组均通过结扎下肢静脉的方法构建下肢DVT模型;建模成功后按照各组给药剂量进行灌胃处理,持续灌胃14 d,模型组及对照组灌胃等量蒸馏水;以苏木精-伊红染法检测大鼠血栓形成处静脉组织病理学变化;全自动血凝仪测定大鼠凝血相关指标;全自动血液流变学仪测定大鼠血液流变相关指标;以酶联免疫吸附试剂盒检测大鼠血栓形成处静脉组织中炎症、氧化应激相关指标表达;蛋白免疫印迹法检测血栓形成处静脉组织中Notch2/Hes-1通路相关蛋白表达。结果:与对照组相比,模型组大鼠活动量减少、双足肿胀、下肢皮肤青紫,股静脉血管中有紫黑色物质存在,血管壁呈现炎性浸润,D-二聚体水平(D-dimer)、血浆黏度、纤维蛋白、红细胞压积显著升高(P<0.05),凝血酶时间(TT)、活化部分凝血活酶时间(APTT)显著降低(P<0.05),血管中炎症因子、丙二醛(MDA)、Notch2、Hes-1蛋白表达水平显著增多(P<0.05),谷胱甘肽过氧化酶(GSH-Px)、超氧化物歧化酶(SOD)表达水平显著降低(P<0.05);与模型组相比,活血灵方各剂量组大鼠血栓形成情况、APTT、TT、血浆黏度、炎性反应、氧化应激反应等均得到一定的缓解(P<0.05),Notch2、Hes-1蛋白表达水平显著降低(P<0.05)。结论:活血灵方缓解大鼠下肢DVT的作用可能是通过抑制Notch2/Hes-1通路实现。Objective:To investigate the effect of Huoxueling Formula on deep venous thrombosis(DVT)of lower limbs and Notch2/Hes-1 pathway in rats.Methods:A total of 50 SD rats were randomly divided into the control group,the model group,the Huoxueling Formula groups of low-dose(6.3 g/kg),medium-dose(12.6 g/kg)and high-dose(25.2 g/kg),with 10 rats in each group.The control group only sutured the wound,however DVT model of the lower limbs was established by ligating the veins of the lower limbs in the other groups.After modeling,the rats in Huoxueling Formula groups were intervened at different dosages for 14 days,and the model group and the control group were only given the same amount of distilled water.The histopathological changes of vein at the thrombosis site were detected by HE staining;the coagulation related indexes were measured by automatic hemagglutination instrument;hemorheology related indexes were measured by automatic hemorheology instrument;the expressions of inflammatory and oxidative stress related indicators in venous tissue of rats with thrombosis were detected by ELISA method;the expressions of Notch2/Hes-1 pathway related proteins were detected by Western blot method.Results:Compared to those in the control group,the activity of the model group was decreased,the feet were swollen,the skin of the lower limbs was blue and purple,there were purple black substances in the femoral vein vessels,and the vascular wall showed inflammatory infiltration,also D-dimer,plasma viscosity,fibrin and hematocrit were significantly increased(P<0.05),but thrombin time(TT)and activated partial thromboplastin time(APTT)were significantly decreased(P<0.05).The expression levels of inflammatory factors,malondialdehyde(MDA),as well as the protein expressions of Notch2 and Hes-1 were significantly increased(P<0.05),and the expression levels of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)were significantly decreased(P<0.05).Compared to those in the model group,the thrombosis,APTT,TT,plasma viscosity,inflammatory re

关 键 词：活血灵方 深静脉血栓 Notch2/Hes-1 

分 类 号：R285.5[医药卫生—中药学]