Biodegradable Polypeptide-based Vesicles with Intrinsic Blue Fluorescence for Antibacterial Visualization  

作　　者：Yu-Ying Yang Ling-Shan Chen Min Sun Cong-Yu Wang Zhen Fan Jian-Zhong Du 

机构地区：[1]Department of Orthopedics,Shanghai Tenth People's Hospital,Tongji University,Shanghai 200072,China [2]Department of Polymeric Materials,School of Materials Science and Engineering,Tongji University,Shanghai 201804,China [3]Institute for Advanced Study,Tongji University,Shanghai 200092,China

出　　处：《Chinese Journal of Polymer Science》2021年第11期1412-1420,共9页高分子科学（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.51803152,22075212 and 21925505);the program for professor of special appointment(Eastern Scholar)at Shanghai institutions of higher learning;Natural Science Foundation of Shanghai(No.19ZR1478800);National Science Fund for Distinguished Young Scholars。

摘　　要：Fluorescence imaging has been an indispensable tool to provide dynamic information about the localization and quantity of organisms.Meanwhile,due to the intrinsic hollow structure and modularized biofunctionalities,polymer vesicles have been widely applied in biomedical field.However,most polymer vesicles are embedded with organic fluorophores for fluorescence imaging,which have certain drawbacks such as leakage and possible cytotoxicity.Here,we present a biodegradable polypeptide-based vesicle with intrinsic blue fluorescence without introducing any fluorophore for real-time visualization of antibacterial process.Through modular design to integrate multiple functional fragments,poly(ε-caprolactone)-block-poly(tryptophan)-block-poly(lysine-stat-phenylalanine)[PCL25-b-PTrp2-b-P(Lys13-statPhe4)]was synthesized,where PCL chains form the hydrophobic membrane,P(Lys-stat-Phe)and PTrp provide intrinsic fluorescence and broadspectrum antibacterial activity.It is noteworthy that the fluorescence emission was shifted from invisible ultraviolet range of amino acids to visible range(emission maximum at 436 nm),which makes it possible to visualize the antibacterial process.In addition,through utilizing the intrinsic fluorescence of vesicles,confocal fluorescent imaging of vesicles with bacteria validated the specific adhesion of vesicle towards bacteria,and the bacterial death through membrane disruption.Overall,we provided a novel approach to developing biodegradable fluorescent polypeptide-based vesicles for real-time visualization of antibacterial process.

关 键 词：Polypeptide-based vesicles Intrinsic fluorescence Antibacterial visualization 

分 类 号：R318.08[医药卫生—生物医学工程] O657.3[医药卫生—基础医学]