高丹草低氢氰酸含量性状主效QTL PA7-2的精细定位  

Fine mapping of main QTL PA7-2 for low hydrocyanic acid content in sorghum-sudangrass hybrid

作　　者：吴国芳 于卓[1] 于肖夏[1] 杨东升[1] 卢倩倩 李景伟 李佳奇 WU Guo-fang;YU zhuo;YU Xiao-xia;YANG Dong-sheng;LU Qian-qian;LI Jing-wei;LI Jia-qi(Agricultural College, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China)

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019

出　　处：《草地学报》2021年第10期2149-2157,共9页Acta Agrestia Sinica

基　　金：国家自然科学基金面上项目(31772664)资助。

摘　　要：为了精细定位高丹草低氢氰酸含量性状主效数量性状基因座QTL PA7-2,对进一步开展低氰性状相关候选基因挖掘、功能解析及分子标记辅助育种等研究提供理论依据。本试验在前期研究工作基础上,从高丹草(散穗高粱Scattered ear Sorghum bicolor×红壳苏丹草Red shell Sorghum sudanense)F2代1200个分离群体单株中筛选出121个QIRs(Quantitative trait locus(QTL)isogenic recombinants)植株,选出低氰和高氰极端株套袋自交获得F3代分离群体,选出QIRs群体植株130个,利用BSA-SSR(Bulked segregation analysis(BSA)and simple sequence repeats)技术构建了长度为230.7 cM、密度为4.81 cM的遗传连锁图谱,通过定位分析明确了QTL PA7-2的位置在38 cM处,位于SSR标记Sobic.8 g1-600和XM00242-400之间。经与高粱基因组比对分析,首次将低氰QTL PA7-2精细定位至高粱8号染色体3.77 Mb(51.415 Mb~55.182 Mb)的物理区间,并发现SSR标记SORBI4G3-600与其紧密连锁。Main quantitative trait locus(QTL)PA7-2 controls low hydrocyanic acid content trait of sorghum-sudangrass hybrid,and fine mapping of this gene can provide the basis of further research on candidate genes related to low-cyanide trait,function analysis,and molecular marker-assisted breeding.Based on our previous research work,a total of 121 quantitative trait loci isogenic recombinants(QIRs)were selected from the F2 segregation population containing 1200 individual plants of sorghum-sudangrass hybrid(Scattered ear Sorghum bicolor×Red shell Sorghum sudanense)in this study.Plants with very low or high cyanide traits were bagged and self-crossed to obtain an F3 segregation population,and from which 130 QIRs individual strains were screened.A genetic linkage map was constructed using bulk segregate analysis and simple sequence repeat markers(BSA-SSR),which covered a total length of 230.7 cM,with an average marker distance of 4.81 cM.The QTL PA7-2 was located at 38 cM,and between SSR markers Sobic.8 g1-600 and XM00242-400.Comparing with the sorghum genome,the low-cyanide QTL PA7-2 was finely mapped at the physical interval of 3.77 Mb(51.415 Mb-55.182 Mb)of chromosome 8 in the sorghum genome for the first time,and an SSR marker SORBI4G3-600 was closely linked with it.

关 键 词：高丹草 低氢氰酸含量性状 主效QTL PA7-2 BSA-SSR技术 精细定位 

分 类 号：S431.4[农业科学—农业昆虫与害虫防治]