温度胁迫对湖北钉螺部分呼吸酶的影响  

Effects of temperature stress on some respiratory enzymes in Oncomelania hupensis

作　　者：潘中武[1] 桂琳[1] 胡霞 章琴 周书林[3] PAN Zhong-wu;GUI Lin;HU Xia;ZHANG Qin;ZHOU Shu-lin(Department of Microbiology and Immunology,Wannan Medical College,Wuhu 241002,Anhui Province,China;Department of Human Anatomy,Anhui College of Traditional Chinese Medicine;Department of Medical Parasitology,Wannan Medical College)

机构地区：[1]皖南医学院医学微生物与免疫学教研室,安徽芜湖241002 [2]安徽中医药高等专科学校解剖学教研室 [3]皖南医学院医学寄生虫学教研室

出　　处：《热带病与寄生虫学》2021年第5期264-265,269,共3页Journal of Tropical Diseases and Parasitology

基　　金：安徽省自然科学基金项目(11040606M214);安徽省教育厅高校自然科学研究重点项目(KJ2017A251)。

摘　　要：目的探索温度胁迫(高温和低温)情况下湖北钉螺体内细胞色素氧化酶和琥珀酸脱氢酶两种酶的含量变化情况。方法将钉螺随机分成对照组、低温组和高温组,分别放置于20℃、4℃和37℃环境中,24 h后取钉螺软体组织,测定各组钉螺细胞色素氧化酶和琥珀酸脱氢酶的含量。结果对照组、低温组和高温组三组钉螺软体组织中细胞色素氧化酶含量分别为(27.11±2.31)U/L、(21.90±0.32)U/L和(21.42±1.84)U/L,三组间不全相同,且差异有统计学意义(F=10.145,P<0.05);琥珀酸脱氢酶含量分别为(1.30±0.002)U/L、(1.24±0.05)U/L和(1.12±0.02)U/L,三组间不全相同,且差异有统计学意义(F=124.624,P<0.05)。结论湖北钉螺在温度胁迫状态下,其软体组织中细胞色素氧化酶和琥珀酸脱氢酶的含量均降低。Objective To investigate the changes of cytochrome oxidase(COX)and succinate dehydrogenase(SDH)contents in Oncomelania hupensis(O.hupensis)under laboratory condition and temperature stress(high temperature and low temperature).Methods O.hupensis snails were randomly divided into control group(T=20℃),low temperature group(T=4℃)and high temperature group(T=37℃).Soft tissues of the snails were taken at 24 h after treatment by the different temperature to measure the changes of COX and SDH level in each group.Results The content of COX and SDH in the soft tissues was(27.11±2.31)U/L,(21.90±0.32)U/L and(21.42±1.84)U/L;(1.30±0.002)U/L,(1.24±0.05)U/L and(1.12±0.02)U/L,respectively for the control,low temperature group and high temperature group.The difference was significant among groups(F=10.145,P<0.05 for COX;F=124.624,P<0.05 for SDH).Conclusion COX and SDH contents tend to decrease in the soft tissue of O.hupensis under temperature stress.

关 键 词：湖北钉螺 温度胁迫 细胞色素氧化酶 琥珀酸脱氢酶 

分 类 号：R184.38[医药卫生—流行病学]