Engineered polymer nanoparticles incorporating L-amino acid groups as affinity reagents for fibrinogen  

作　　者：Yongyan Zhu Ruixuan Liu Dengyu Wu Qianqian Yu Kenneth J.Shea Quanhong Zhu 

机构地区：[1]School of Traditional Chinese Medicine,Southern Medical University,Guangzhou,510515,China [2]Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics,Guangzhou,510515,China [3]Guangdong Provincial Engineering Laboratory of Chinese Medicine Preparation Technology,Guangzhou,510515,China [4]Department of Chemistry,University of California,Irvine,CA,92697,USA

出　　处：《Journal of Pharmaceutical Analysis》2021年第5期596-602,共7页药物分析学报（英文版）

基　　金：This work was supported by the Natural Science Foundation of Guangdong Province,China(Grant No.:2017A030313775);the Science and Technology Planning Project of Guangdong Province,China(Grant No.:2016A010103016);the Science and Technology Planning Project of Guangzhou City of Guangdong Province,China(Grant No.:201607010148).

摘　　要：Synthetic polymer hydrogel nanoparticles(NPs)were developed to function as abiotic affinity reagents for fibrinogen.These NPs were made using both temperature-sensitive N-isopropyl acrylamide(NIPAm)and L-amino acid monomers.Five kinds of L-amino acids were acryloylated to obtain functional monomers:L-phenylalanine(Phe)and L-leucine(Leu)with hydrophobic side chains,L-glutamic acid(Glu)with negative charges,and L-lysine(Lys)and L-arginine(Arg)with positive charges.After incubating the NPs with fibrinogen,g-globulin,and human serum albumin(HSA)respectively,the NPs that incorporated Nacryloyl-Arg monomers(AArg@NPs)showed the strongest and most specific binding affinity to fibrinogen,when compared with g-globulin and HSA.Additionally,the fibrinogen-AArg binding model had the best docking scores,and this may be due to the interaction of positively charged AArg@NPs and the negatively charged fibrinogen D domain and the hydrophobic interaction between them.The specific adsorption of AArg@NPs to fibrinogen was also confirmed by the immunoprecipitation assay,as the AArg@NPs selectively trapped the fibrinogen from a human plasma protein mixture.AArg@NPs had a strong selectivity for,and specificity to,fibrinogen and may be developed as a potential human fibrinogen-specific affinity reagent.

关 键 词：Synthetic polymer nanoparticles Amino-acid monomers ARGININE FIBRINOGEN Affinity reagent Protein interaction 

分 类 号：R318.08[医药卫生—生物医学工程] TB383.1[医药卫生—基础医学]