Electrochemical detection of methyl-paraoxon based on bifunctional cerium oxide nanozyme with catalytic activity and signal amplification effect  

作　　者：Yuzhou Sun Jinchao Wei Jian Zou Zehua Cheng Zhongming Huang Liqiang Gu Zhangfeng Zhong Shengliang Li Yitao Wang Peng Li 

机构地区：[1]State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macao,Macao,999078,China [2]Institute of Traditional Chinese Medicine&Natural Products,College of Pharmacy,Jinan University,Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research,Guangzhou,510632,China [3]Integrated Chinese and Western Medicine Postdoctoral Research Station,Jinan University,Guangzhou,510632,China [4]Center of Super-Diamond and Advanced Films(COSDAF)and Department of Chemistry,City University of Hong Kong,Hong Kong,999077,China

出　　处：《Journal of Pharmaceutical Analysis》2021年第5期653-660,共8页药物分析学报（英文版）

基　　金：This work was supported by Macao Science and Technology Development Fund(Grant No.:0147/2019/A3);Guangxi Innovation-driven Development Special Foundation Project(Project No.:GuiKe AA18118049);China Postdoctoral Science Foundation(Grant No.:2019M653299);the National Natural Science Foundation of China(Grant No.:81903794).

摘　　要：A new electrochemical sensor for organophosphate pesticide(methyl-paraoxon)detection based on bifunctional cerium oxide(CeO_(2))nanozyme is here reported for the first time.Methyl-paraoxon was degraded into p-nitrophenol by using CeO_(2) with phosphatase mimicking activity.The CeO_(2) nanozymemodified electrode was then synthesized to detect p-nitrophenol.Cyclic voltammetry was applied to investigate the electrochemical behavior of the modified electrode,which indicates that the signal enhancement effect may attribute to the coating of CeO_(2) nanozyme.The current research also studied and discussed the main parameters affecting the analytical signal,including accumulation potential,accumulation time,and pH.Under the optimum conditions,the present method provided a wider linear range from 0.1 to 100 mmol/L for methyl-paraoxon with a detection limit of 0.06 mmol/L.To validate the proof of concept,the electrochemical sensor was then successfully applied for the determination of methyl-paraoxon in three herb samples,i.e.,Coix lacryma-jobi,Adenophora stricta and Semen nelumbinis.Our findings may provide new insights into the application of bifunctional nanozyme in electrochemical detection of organophosphorus pesticide.

关 键 词：Chinese medicine Nanozyme ORGANOPHOSPHORUS Pesticide Methyl-paraoxon ELECTROANALYSIS 

分 类 号：O657.1[理学—分析化学] R284.1[理学—化学]