魔芋软腐病拮抗放线菌筛选  

Screening of Antagonistic Actinomycetes Against Amorphophallus Soft Rot

作　　者：代雪凤 朱丽 张盛林[1,2] 牛义 刘海利[1,2] DAI Xuefeng;ZHU Li;ZHANG Shenglin;NIU Yi;LIU Haili(School of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China;Chongqing Key Laboratory of Olericalture,Chongqing 400715,China)

机构地区：[1]西南大学园艺园林学院,重庆400715 [2]重庆市蔬菜学重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2021年第11期9-17,共9页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市技术创新与应用发展专项面上项目(cstc2019jscx-msxmX0404).

摘　　要：软腐病是魔芋一种毁灭性的细菌性病害,致病菌多样,防治困难.为寻求新的防治途径,以致病力强的软腐病菌株MY-30(Pectobacterium carotovorum subsp.carotovorum)为靶菌,采用双层培养基法等方法筛选出对魔芋软腐病菌具有良好拮抗作用的放线菌,并对其生长特性、抑菌广谱性等进行分析.结果表明:26株放线菌对软腐菌MY-30有抑菌活性,其中菌株F188抑菌效果最好,其发酵原液和发酵滤液对离体魔芋球茎软腐病菌的防效为62.5%,抑菌谱广,对魔芋的生长具有一定的促进作用.放线菌F188可用作魔芋软腐病的生防菌种,具有良好的应用前景.Soft rot is a devastating bacterial disease of Amorphophallus.The pathogenic bacteria are diverse and difficult to control.In order to find a new way of prevention and control,the strongly pathogenic soft rot strain MY-30(Pectobacterium carotovorum subsp.carotovorum)was used as the target bacteria,and the double-layer medium method was used to screen out Actinomycetes with good antagonistic effect against Amorphophallus soft rot,and analyze their growth characteristics and broad-spectrum bacteriostasis.Twenty-six strains of actinomycetes showed antibacterial activity against MY-30,of which strain F188 was the best.Its fermentation stock and fermentation filtrate had a control effectiveness of 62.5%against isolated Amorphophallus bulb soft rot and had a broad antibacterial spectrum.It had a certain promoting effect on the growth of Amorphophallus as well.Actinomycetes F188,therefore,is recommended as a biocontrol strain for Amorphophallus soft rot.

关 键 词：魔芋软腐病 病原菌 拮抗放线菌 抗菌谱 生物防效 

分 类 号：S432.4[农业科学—植物病理学]