IL-21在骨性关节中的作用及机制研究  

The role and mechanism of IL-21 in knee osseous joint

作　　者：魏松松 徐斌[1] 汪昊 涂俊[1] 王瑞[1] 姜少伟[1] 张珂 Wei Singsong;Xu Bin;Wang Hao(Dept of Sports Trauma and Arthroscopic Surgery,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院运动创伤与关节镜外科,合肥230022

出　　处：《安徽医科大学学报》2021年第11期1829-1833,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1808085MH243)。

摘　　要：通过ELISA技术分析骨关节炎(OA)患者与正常对照组外周血及关节微环境中白介素21(IL-21)的变化;应用流式细胞术检测OA患者和正常对照者外周血单个核细胞中CD3^(+)CD8^(-)IL-21^(+)T淋巴细胞及关节滑膜中浸润的CD3^(+)CD8^(-)IL-21^(+)T淋巴细胞的百分比例变化。与正常对照者相比,OA患者的血清及关节微环境中IL-21浓度升高,并且患者的外周血单个核细胞中CD3^(+)CD8^(-)IL-21^(+)T细胞占总淋巴细胞的百分比增加,同时OA患者滑膜浸润的淋巴细胞中CD3^(+)CD8^(-)IL-21^(+)T细胞百分比也增加。实验表明在OA患者中IL-21的表达量增加,并可能通过增加异常的免疫级联反应参与该疾病的发病机制。The purpose of the experiment was to analyze the changes of interleukin-21(IL-21)in peripheral blood samples and joint microenvironment of patients with osteoarthritis(OA)and healthy controls by ELISA,and to detect the percentage of CD3^(+)CD8^(-)IL-21^(+)T lymphocytes in peripheral blood mononuclear cells and infiltrating CD3^(+)CD8^(-)IL-21^(+)T lymphocytes in synovium of OA patients and healthy controls by flow cytometry.Compared with healthy volunteers,the concentration of IL-21 in serum and joint microenvironment of OA patients increased,and the percentage of CD3^(+)CD8^(-)IL-21^(+)T cells in peripheral blood mononuclear cells in total lymphocytes increased.At the same time,the percentage of CD3^(+)CD8^(-)IL-21^(+)T cells in synovial infiltrating lymphocytes in OA patients also increased.It was found that the expression of IL-21 increased in patients with OA,and might be involved in the pathogenesis of the disease by increasing abnormal immune cascade reaction.

关 键 词：膝关节 骨性关节炎 白介素21 流式细胞术 

分 类 号：R684.3[医药卫生—骨科学] R392.12[医药卫生—外科学]