不同产地丹参及根际土壤无机元素分析与评价  

Analysis and Evaluation of Inorganic Elements in Salvia miltiorrhiza and Rhizosphere Soils From Different Areas

作　　者：刘沁荣 杜紫微 李佳珍 王一硕[1,3] 顾选 崔秀梅 LIU Qin-rong;DU Zi-wei;LI Jia-zhen;WANG Yi-shuo;GU Xuan;CUI Xiu-mei(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Beijing Huamiao Pharmaceutical Co.,Ltd.,Beijing 102600,China;Henan Province TCM Production Integration Engineering Technology Research Center,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]国药集团北京华邈药业有限公司,北京102600 [3]河南省中药生产一体化工程技术研究中心,河南郑州450046

出　　处：《光谱学与光谱分析》2021年第11期3618-3624,共7页Spectroscopy and Spectral Analysis

基　　金：国家中医药管理局公益性行业专项(00104296);河南省科技创新杰出青年基金项目(184100510017)资助。

摘　　要：无机元素是自然界植物生长过程中所必需的物质,也是中药材的基本组成成分,在中药质量控制和评价应用中是不可或缺的参数。原子吸收分光光度技术作为无机元素测定方法,在中草药及成药分析中起到重要的作用。根际是植物和土壤进行物质能量交换的结点,根际土壤中营养元素与中药材内在品质密切相关。研究以5个省份9个主产区的丹参及根际土壤为样品,采用原子吸收分光光度法对样品中Na, Mg, K, Ca, Mn, Fe, Cu和Zn八种无机元素含量进行检测,采用聚类分析、主成分分析和偏最小二乘判别分析等化学模式识别方法进行探讨。结果表明:建立的原子吸收分光光度方法线性关系良好,具有较高的准确度和精密度;比较各产地样品无机元素含量,发现山东丹参中Mn元素含量颇高,四川丹参中Fe和K元素含量较高,陕西丹参根际土壤中Ca元素含量较高;聚类分析显示不同产地丹参存在明显差异,且根际土壤中的K, Na, Mn和Zn元素与药材中多个无机元素呈现相关关系;主成分分析结果表明土壤中元素影响着药材成分元素含量若以这八种元素为评价指标山东产区丹参质量较佳;偏最小二乘判别分析结果发现Na, K, Fe和Mg四个元素可能是造成不同产地丹参质量差异的主要影响因素。采用原子吸收分光光度技术建立不同产地丹参及根际土壤中无机元素含量方法,探讨道地药材质量与生长环境两者间关系,为丹参药材质量控制及标准建立提供科学参考。Inorganic elements are essential substances in the growth process of plants in nature and are also the basic components of Chinese medicinal materials. Their composition and content determine the efficacy and properties of Chinese medicine and are an indispensable parameter in the quality control and evaluation of Chinese medicine. The rhizosphere is the node of material and energy exchange between plants and soil. The nutrient elements of the rhizosphere soil are closely related to the intrinsic quality of Chinese medicinal materials. The changes in the composition of medicinal materials and the law of action due to differences in soil, production areas and other ecological factors are issues worthy of study. As an important element determination method, atomic absorption spectrophotometry plays an important role in analysing Chinese herbal medicines and finished medicines. The study used samples of Salvia miltiorrhiza and rhizosphere soil from 9 main producing areas in 5 provinces were used. The atomic absorption spectrophotometry was used to detect the contents of eight inorganic elements of Na, Mg, K, Ca, Mn, Fe, Cu and Zn in the samples. Use cluster analysis, principal component analysis, orthogonal partial least square discriminant analysis and other chemical pattern recognition methods to discuss and summarize. The results show that the established atomic absorption spectrophotometric method has a good linear relationship and has high accuracy and precision. The content of Mn in the Salvia miltiorrhiza from the Shan-dong area is higher than that in other producing areas. Salvia miltiorrhiza from the Si-chuan area have higher Fe and K elements, while the content of Ca in the rhizosphere soil of Salvia miltiorrhiza from Shan-xi province is slightly higher. Cluster analysis showed that there were significant differences between different origins of Salvia miltiorrhiza. The K, Na, Mn and Zn elements in the in root soil showed correlations with several elements in the herbs. The results of the principal compone

关 键 词：丹参 根际土壤 原子吸收分光光度法 无机元素 

分 类 号：R282.2[医药卫生—中药学]