基于微流控系统的单细胞代谢物分析技术的研究进展  

Progress of Single-cell Metabolite Analysis Technology Based on Microfluidic System

作　　者：张乃倩 方群[1,2,3] ZHANG Nai-Qian;FANG Qun(Institute of Microanalytical Systems,Department of Chemistry,Zhejiang University,Hangzhou 310058,China;Key Laboratory of Excited-State Materials of Zhejiang Province,Zhejiang University,Hangzhou 310027,China;Hangzhou Innovation Center,Zhejiang University,Hangzhou 311200,China)

机构地区：[1]浙江大学化学系微分析系统研究所,杭州310058 [2]浙江大学浙江省激发态材料合成与应用重点实验室,杭州310027 [3]浙江大学杭州国际科创中心,杭州311200

出　　处：《分析化学》2021年第11期1779-1791,共13页Chinese Journal of Analytical Chemistry

基　　金：国家重大科研仪器研制项目(No.21827806)资助。

摘　　要：由于细胞之间存在异质性,单细胞水平的代谢物分析可为复杂生物系统的研究提供更丰富的信息。微流控系统可在微米级结构内对流体进行操纵,其微结构尺度与细胞大小接近,非常适合进行单细胞分析。本文对基于微流控系统的单细胞代谢物分析技术的进展进行了综述,根据分析中所采用检测方法的不同,分别详细介绍了基于荧光显微成像、激光诱导荧光、电化学、质谱、化学发光和传感检测技术的微流控单细胞代谢物分析系统及其应用研究。Due to the heterogeneity between different cells,the analysis of metabolites at single cell level can provide abundant information for the study of complex biological systems.Microfluidic systems can manipulate fluids in microscale structures that are close to the size of cells,making microfluidic systems suitable for single-cell analysis.In this review,current single-cell metabolite analysis technology based on microfluidic systems was introduced.Various microfluidic single-cell metabolite analysis systems based on different detection techniques including fluorescence microscopic imaging,laser induced fluorescence,electrochemistry,mass spectrometry,chemiluminescence,sensor detection technology,as well as their applications were reviewed.

关 键 词：微流控系统 单细胞分析 代谢物分析 评述 

分 类 号：Q2-33[生物学—细胞生物学] O652[理学—分析化学]