烟草甲幼虫高毒力病原真菌的筛选  

Screening for Highly Virulent Entomopathogenic Fungi Against Lasioderma serricorne(Fabricius)Larvae

作　　者：齐禹哲 闫芳芳[2] 李成军[3] 刘苏[1] 陈鹏 徐传涛 李茂业[1] 刘明科 闫雪梨 李斌 QI Yuzhe;YAN Fangfang;LI Chengjun;LIU Su;CHEN Peng;XU Chuantao;LI Maoye;LIU Mingke;YAN Xueli;LI Bin(Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes,School of Plant Protection,Anhui Agricultural University,Hefei 230036,China;Sichuan Panzhihua Branch Tobacco Company,Panzhihua,Sichuan 617026,China;Tobacco Research Institute of Henan Academy of Agricultural Sciences,Xuchang,Henan 464000,China;Sichuan Branch of China National Tobacco Corporation,Chengdu 630041,China;Luzhou Company of Sichuan Tobacco Company,Luzhou,Sichuan 646600,China)

机构地区：[1]安徽农业大学植物保护学院,植物病虫害生物学与绿色防控安徽普通高校重点实验室,合肥230036 [2]四川省烟草公司攀枝花市公司,四川攀枝花617026 [3]河南省农业科学院烟草研究所,河南许昌464000 [4]中国烟草总公司四川省公司,成都630041 [5]四川省烟草公司泸州市公司,四川泸州646600

出　　处：《中国烟草科学》2021年第5期57-62,68,共7页Chinese Tobacco Science

基　　金：中国烟草总公司四川省公司重点科技项目(SCYC201806);四川省烟草公司攀枝花市公司重点项目(SCYC201802)。

摘　　要：为筛选出对烟草甲2龄幼虫具有较好防治效果的病原真菌,在实验室条件下,采用浸渍法测定5株蜡蚧轮枝菌和5株球孢白僵菌对烟草甲2龄幼虫的毒力,筛选出2株对烟草甲2龄幼虫具有高毒力的白僵菌菌株Bb05和Bbr81,将2株菌株配置成不同浓度的孢子悬浮液,测定其毒力并对Bb05菌株的侵染过程进行观察。结果表明,接种孢子浓度1.0×10^(8)mL^(-1)条件下,菌株Bb05处理烟草甲的累积死亡率最高,达77.78%,LT_(50)为8.18 d;菌株Bbr81处理烟草甲的累积死亡率为74.44%,LT_(50)为7.60 d。用不同浓度孢子悬浮液处理烟草甲2龄幼虫后,其累积死亡率随孢子浓度和接种时间的增加而增加,Bb05菌株的LC50为8.3×10^(7)mL^(-1),菌株Bbr81的LC50为1.2×10^(7)mL^(-1)。筛选所得的2个菌株对烟草甲虫2龄幼虫的毒力较高,具有一定的开发应用潜力。In order to screen for entomopathogenic fungi with better control effect on the second instar larvae of Lasioderma serricorne,in this experiment,5 strains of Verticillium lecanii and 5 strains of Beauveria bassiana were determined by the impregnation method.Two Beauveria bassiana strains Bb05 and Bbr81 with high virulence to the second instar larvae of Lasioderma serricorne were obtained,and the two strains were prepared into different concentrations of spore suspension,and their virulence was determined.The infection process of the Bb05 strain was observed.The results showed that,at the concentration of 1.0×10^(8)mL^(-1),Bb05 had the highest cumulative death rate to L.serricorne(77.78%)and LT_(50)(8.18 d).The cumulative mortality of strain Bbr81 and LT_(50)was 74.44%and 7.60 d respectively.After treating the Lasioderma serricorne with different concentrations of spore suspensions,the cumulative mortality increased with the increase of spore concentration and inoculation time.The LC_(50)of strain BB05 was 8.3×10^(7)spores mL^(-1),and that of strain Bbr81 was 1.2×10^(7)spores mL^(-1).The two strains were highly virulent to the 2nd instar larvae of tobacco beetle,and have a certain potential for development and application.

关 键 词：昆虫病原真菌 烟草甲 生物测定 生物防治 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] S476.12[农业科学—植物保护]