高速逆流色谱法分离纯化大风艾中的黄酮类成分  

Isolation and purification of flavonoid glycosides from Blumea balsamifera using high-speed counter-current chromatography

作　　者：谭道鹏 郭振虎 何莉 秦琳 何芋岐 Tan Daopeng;Guo Zhenhu;He Li;Qin Lin;He Yuqi(Key Laboratory of Basic Pharmacalogy of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education,School of Pharmacy,Zunyi Medical University,Zunyi Guizhou 563009,China;State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang Guizhou 550014,China;Guizhou Huangguoshu Lishuang Pharmaceutcial Co.Ltd,Guiyang Guizhou 550014,China;Guizhou Miaoyao Biotechnology Co.Ltd,Tongren Guizhou 554300,China)

机构地区：[1]遵义医科大学药学院,贵州遵义563009 [2]贵州医科大学省部共建药用植物功效与利用国家重点实验室,贵州贵阳550014 [3]贵州黄果树立爽药业有限公司,贵州贵阳550014 [4]贵州苗药生物技术有限公司,贵州铜仁554300

出　　处：《遵义医科大学学报》2021年第4期535-539,共5页Journal of Zunyi Medical University

基　　金：贵州省科技计划项目(NO:QKHZC[2021]476,QKHZC[2019]2961,QKHPTRC[2019]5657,QKHPTRC[2018]5772-001,QKHZDZXZ[2019]3001,QKHZC[2019]2953,QKHZC[2020]4Y072,QKHZC[2021]420,QKHZC[2019]2829);贵州省教育厅重点领域项目(NO:QJHKY[2021]049);贵州医科大学药用植物功效利用国家重点实验室资助项目(NO:FAMP201909K)。

摘　　要：目的建立分离纯化大风艾中黄酮苷类成分的方法。方法目标成分可以通过高速逆流色谱,以水-甲醇-乙酸乙酯-正己烷(5∶1∶5∶1,v/v/v/v)两相溶剂系统进行分离。结果经一次分离纯化可获得9.3 mg金丝桃苷(纯度97.1%)、5.7 mg紫云英苷(纯度82.8%)和7.6 mg槲皮苷(纯度98.8%),以上3个化合物经ESI-MS,1H NMR鉴定。结论本研究建立的方法可用于大风艾中黄酮苷类成分的分离和纯化。Objective To isolate the flavonoid glycosides in the extract of Blumea balsamifera.Methods The target constituents were separated by high-speed counter-current chromatography(HSCCC)using water-methanol-ethyl acetate-n-hexane(5∶1∶5∶1,v/v/v/v)solvent system.Results A total of 9.3 mg of hyperoside,5.7 mg of astragalin and 7.6 mg of quercitrin with the purity of 97.1%,82.8%and 98.8%were isolated and purified,respectively.The structure of those three compounds were identified by NMR,ESI-MS.Conclusion The established methods could be employed to isolate the flavonoid glycosides in the extract of Blumea balsamifera.

关 键 词：高速逆流色谱 分离纯化 大风艾 黄酮 

分 类 号：R282.5[医药卫生—中药学]