卡介苗多糖核酸和OVA过敏原复合壳聚糖纳米缓释疫苗对哮喘小鼠肺泡灌洗液中炎症细胞及细胞因子的影响  

Effects of BCG-polysaccharide nucleic acid and OVA allergen combined with chitosan nano sustained-release vaccine on inflammatory cells and cytokines in bronchoalveolar lavage fluid of asthmatic mice

作　　者：杨文涛[1] 谢艳萍[1] YANG Wentao;XIE Yanping(Department of Respiratory Medicine,Huzhou First People′s Hospital in Zhejiang Province,Huzhou313000,China)

机构地区：[1]浙江省湖州市第一人民医院呼吸内科,浙江湖州313000

出　　处：《中国现代医生》2021年第25期43-46,共4页China Modern Doctor

基　　金：浙江省科技计划项目(2017C37103);浙江省湖州市科学技术局公益性应用研究项目(2017GYB43)。

摘　　要：目的探讨卡介苗多糖核酸(BCG-PSN)和卵蛋白(OVA)过敏原复合壳聚糖(CS)纳米缓释疫苗对哮喘小鼠支气管肺泡灌洗液(Bronchoalveolar lavage fluid,BALF)中炎症细胞及炎症因子的影响。方法将80只实验小鼠随机平均分为八组(A组:正常对照组;B组:哮喘组;C组:空白壳聚糖组;D线:CS-BCG-PSN组;E组:CS-OVA组;F组:BCG-PSN组;G组:CS-BCG-PSN-OVA疫苗组;H组:激素组)。除正常对照组和哮喘组外,各组均于第18天和第24天腹腔注射相应药物干预哮喘小鼠。激素组小鼠于雾化激发哮喘前腹腔内注射地塞米松针。哮喘组、正常对照组在各个时间点给予相应剂量的生理盐水。末次激发后48 h,测定BALF中相关细胞因子(IL-4、IFN-γ)及白细胞和嗜酸性粒细胞的计数。结果D组、E组、F组、G组白细胞及嗜酸性粒细胞数、IL-4均低于B组及C组,壳聚糖同时包埋卡介苗多糖核酸、卵蛋白作用于哮喘小鼠后,白细胞、嗜酸性粒细胞数及IL-4下降的程度,较单独包埋卡介苗多糖核酸及卵蛋白更大(P<0.05);同时,D组、E组、F组、G组IFN-γ均高于哮喘组及空白壳聚糖组,壳聚糖同时包埋卡介苗多糖核酸、卵蛋白作用于哮喘小鼠后,IFN-γ的升高程度较单独包埋卡介苗多糖核酸及卵蛋白更大(P<0.05)。结论CS-BCG-PSN-OVA复合物可以调节细胞数及细胞因子的水平,并可能通过此来纠正Th1/Th2比例失衡,并进一步缓解气道炎症,为哮喘治疗提供理论依据。Objective To explore the effects of BCG-polysaccharide nucleic acid(BCG-PSN)and ovalbumin(OVA)allergen combined with chitosan(CS)nano sustained-release vaccine on inflammatory cells and inflammatory factors in bronchoalveolar lavage fluid(BALF)of asthmatic mice.Methods A total of 80 mice were randomly divided into 8 groups(group A was normal control group,group B was asthma group,group C was blank CS group,group D was CS-BCG-PSN group,group E was CS-OVA group,group F was BCG-PSN group,group G was CS-BCG-PSN-OVA vaccine group,group H was hormone group).Except for the normal control group and asthma group,asthmatic mice in each group received intraperitoneal injection of corresponding drugs on the 18th and 24th day.The mice in the hormone group were injected with dexamethasone intraperitoneally before atomization-induced asthma.The asthma group and the normal control group were given the corresponding dose of normal saline at each time point.48 hours after the last induction,the related cytokines(IL-4 and IFN-γ)and the count of leukocytes and eosinophils in BALF were measured.Results The number of leukocytes and eosinophils and IL-4 in group D,group E,group F and group G were lower than those in group B and group C.The decrease of leukocyte,eosinophil count and IL-4 in asthmatic mice treated with CS was more significant than that in BCG-PSN and OVA alone(P<0.05).Moreover,the levels of IFN-γin group D,E,F and G was higher than that in asthma group and blank CS group,and the increase of IFN-γin asthmatic mice treated with BCG-PSN combined with OVA was higher than that in BCG-PSN and OVA separately(P<0.05).Conclusion CS-BCG-PSN-OVA complex can regulate the number of cells and the level of cytokines,which may be used to correct the imbalance of Th1/Th2 proportion and further alleviate airway inflammation,thereby providing a theoretical basis for the treatment of asthma.

关 键 词：卡介苗多糖核酸 卵蛋白 哮喘 细胞因子 

分 类 号：R186[医药卫生—流行病学]