甘蓝型油菜BnTLP1基因的菌核病抗性研究  

BnTLP1 on resistance to Sclerotinia sclerotiorum in Brassica napus

作　　者：石美娟 左蓉 刘杰 赵传纪 董志雪 何贻洲 李艳 李祥[1] 童超波[1] 黄邦全[2] 白泽涛[1] 刘胜毅[1,2] SHI Mei-juan;ZUO Rong;LIU Jie;ZHAO Chuan-ji;DONG Zhi-xue;HE Yi-zhou;LI Yan;LI Xiang;TONG Chao-bo;HUANG Bang-quan;BAI Ze-tao;LIU Sheng-yi(Oil Crops Research Institute of Chinese Academy of Agriculture Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China;Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources,Hubei University,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所,农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]湖北大学生物资源绿色转化湖北省协同创新中心,湖北武汉430062

出　　处：《中国油料作物学报》2021年第5期752-761,共10页Chinese Journal of Oil Crop Sciences

基　　金：国家重点研发计划项目(2016YFD0101007);国家自然科学基金(31770250)。

摘　　要：前期发现甘蓝型油菜类甜蛋白基因BnTLP1在菌核病抗性品种中的表达丰度高,为探究和解析其作用机制,通过叶片接种核盘菌,对拟南芥BnTLP1过表达转基因株系、野生型对照和突变体tlp1-1之间的抗菌核病效果进行分析。结果表明:过表达BnTLP1拟南芥的病斑面积显著小于野生型对照,而tlp1-1病斑扩展大于对照,即BnTLP1正调控菌核病抗性。亚细胞定位表明BnTLP1定位于细胞膜和细胞核,暗示其不仅在细胞膜发挥抑菌功能,而且在核内行使其它功能。不同防御反应信号通路的标志基因表达分析表明,茉莉酸/乙烯途径可能在早期参与了BnTLP1介导的菌核病抗性。BnTLP1 gene was previously found highly expressed in tolerant Brassica napus cv.Zhongyou 821.To deep understand the tolerance to Sclerotinia sclerotiorum,BnTLP1 gene was identified using overexpressed transgenic Arabidopsis lines.Leaf inoculation was carried out using BnTLP1 overexpression transgenic Arabidopsis lines,wild-type and tlp1-1 mutant by S.sclerotiorum mycelial agar plugs.Compared to WT,BnTLP1 overexpression lines exhibited the smallest lesion,while tlp1-1 showed the largest one,suggesting that BnTLP1 could positively regulated Sclerotinia disease resistance.Subcellular localization indicated that BnTLP1 was located in cell membrane and nucleus.Expression analysis of marker genes in different defense signaling showed that jasmonic acid/ethylene might play roles in the early stage of BnTLP1-mediated diseased resistance.

关 键 词：甘蓝型油菜 BnTLP1基因 核盘菌 转基因拟南芥 亚细胞定位 茉莉酸/乙烯途径 

分 类 号：S565.4[农业科学—作物学] Q789[生物学—分子生物学]