勿动蛋白与Rho相关蛋白激酶对心脏交感神经重塑与去甲肾上腺素转运蛋白表达的调节  

Cardiac sympathetic nerve remodeling and norepinephrine transporter expression regulated by Nogo and Rho-associated protein kinase(ROCK)

作　　者：李世军[1] 李昂[1] 张璐[1] 王晓娜[1] Li Shijun;Li Ang;Zhang Lu(Department of Cardiology,The Second Medical Center,Chinese PLA General Hospital,National Clinical Research Center for Geriatric Diseases,Beijing 100853,China)

机构地区：[1]解放军总医院第二医学中心心血管内科国家老年疾病临床研究中心,北京100853

出　　处：《中华保健医学杂志》2021年第5期434-437,共4页Chinese Journal of Health Care and Medicine

基　　金：国家自然科学基金(81970341)。

摘　　要：目的探讨勿动蛋白(Nogo)与Rho相关蛋白激酶(ROCK)对心脏交感神经重塑与去甲肾上腺素转运蛋白表达的调节作用。方法建立SD乳鼠脊髓背根神经元细胞与心肌细胞联合培养的模型,随机数表法分为A组(心肌细胞+交感神经节细胞);B组(心肌细胞+交感神经节细胞+Nogo-66);C组(心肌细胞+交感神经节细胞+C3转移酶+Nogo-66);D组(心肌细胞+交感神经节细胞+Y-2763+Nogo-66)。倒置相差显微镜观察神经元细胞与心肌细胞共培养细胞的生长状况。免疫荧光测定酪氨酸羟化酶(TH)与去甲肾上腺素转运蛋白表达。ELISA检测培养液中去甲肾上腺素含量(NE)。结果免疫荧光染色结果显示,与A组(1.00±0.00)比较,B组(0.07±0.01)TH表达明显降低,C组(1.99±0.55)与D组(2.13±0.72)TH表达明显增加,差异均有统计学意义(P<0.05)。ELISA检测结果显示,与A组(117.34±2.47)ng/L比较,B组[(94.32±7.34)ng/L]去甲肾上腺素含量明显降低,C组[(124.61±2.97)ng/L)与D组[(127.19±3.54)ng/L)去甲肾上腺素含量明显增加,差异有统计学意义(P<0.05)。免疫荧光染色结果显示,与A组(1.00±0.00)比较,B组(0.07±0.01)TH表达明显降低,C组(1.99±0.55)与D组(2.13±0.72)TH表达明显增加,差异有统计学意义(P<0.05)。。结论Nogo与ROCK参与心脏交感神经重塑与去甲肾上腺素转运蛋白表达的调节。Objective To investigate cardiac sympathetic nerve remodeling and expression of norepinephrine transporter(NET)regulated by Nogo and ROCK.Methods Thirty SD rats aged 1-3 days were used to prepare cardiomyocytes and spinal dorsal root neurons,and establish a model of co-culture of spinal dorsal root neurons and cardiomyocytes.The cell model was randomly divided into four groups:group A:cardiomyocytes and sympathetic ganglion cells;group B:cardiomyocytes,sympathetic ganglion cells and Nogo-66;group C:cardiomyocytes,sympathetic ganglion cells,C3 transferase and Nogo-66;group D:cardiomyocytes,sympathetic ganglion cells,Y-2763 and Nogo-66.Expression of tyrosine hydroxylase(TH)and norepinephrine transporter was detected by immunofluorescence.Norepinephrine(NE)was detected by ELISA.Results Compared with group A(1.00±0.00),expression of TH in group B(0.07±0.01,P=0.035)was significantly lower than that in group A(1.99±0.55,P=0.027)and D(2.13±0.72,P=0.015).Compared with group A(117.34±2.47)ng/L,the norepinephrine content in group B[(94.32±7.34)ng/L,P<0.001]was significantly lower than that in group A(124.61±2.97)ng/L,P=0.021)and D[(127.19±3.54)ng/L,P=0.003].Compared with group A(1.00±0.00),TH expression in group B(0.07±0.01,P=0.035)was significantly lower than that in group A(1.00±0.00),while those in group C(1.99±0.55,P=0.027)and group D(2.13±0.72,P=0.015)were significantly increased.Conclusion Nogo and ROCK are involved in regulation of cardiac sympathetic nerve remodeling and norepinephrine transporter expression.

关 键 词：勿动蛋白 信号通路 心脏交感神经重塑 心去甲肾上腺素转运蛋白 

分 类 号：R54[医药卫生—心血管疾病]