基于CdSe/ZnS量子点构建乙酰甲胺磷荧光检测方法研究  

Fluorometric Determination of Acephate Based on CdSe/ZnS Quantum Dots

作　　者：程杨[1] 刘红弟[1] 匡立学[1] 徐国锋[1] CHENG Yang;LIU Hongdi;KUANG Lixue;XU Guofeng(Institute of Pomology of Chinese Academy of Agricultural Sciences,Supervision and Test Center of Fruit and Nursery Stocks Quality,Ministry of Agriculture and Rural Affairs(Xingcheng),Xingcheng125100)

机构地区：[1]中国农业科学院果树研究所,农业农村部果品及苗木质量监督检验测试中心(兴城),辽宁兴城125100

出　　处：《分析科学学报》2021年第5期606-610,共5页Journal of Analytical Science

基　　金：中国农业科学院创新工程项目(No.CAAS-ZDRW202011);国家农产品质量安全风险评估重大专项(No.GJFP2019011);国家现代农业产业技术体系建设专项资金(No.CARS-27)。

摘　　要：本文以CdSe/ZnS量子点为荧光探针,基于乙酰甲胺磷对CdSe/ZnS量子点的荧光猝灭效应,建立了一种可快速测定乙酰甲胺磷的荧光检测方法。通过透射电子显微镜(TEM)、紫外-可见吸收光谱和荧光光谱对CdSe/ZnS量子点进行表征。在反应时间为5 min条件下,乙酰甲胺磷的浓度在0.487×10^(-6)~7.225×10^(-6)mol/L范围内与CdSe/ZnS量子点的荧光猝灭强度比值呈良好的线性关系(R2=0.9987),方法检出限为2.55×10^(-7)mol/L。在2.0、5.0μmol/L加标水平下的回收率为94.5%~102.8%,相对标准偏差(RSD,n=5)小于4%。该方法选择性好,灵敏度高,可用于水果和蔬菜中农药乙酰甲胺磷的快速检测。Based on the fluorescence quenching effect of acephate on CdSe/ZnS quantum dots(QDs),a fluorometric method for the rapid determination of acephate was established.The CdSe/ZnS QDs were characterized by transmission electron microscopy,UV-Vis absorption spectrum and fluorescence spectrum.When the reaction time was 5 min,the concentration of acephate in the range of 0.487×10^(-6)-7.225×10^(-6)mol/L had a good linear relationship with the fluorescence quenching ratio of CdSe/ZnS QDs(R2=0.9987),and the limits of detection was 2.55×10^(-7)mol/L.The recoveries at the spiked levels of 2.0μmol/L and 5.0μmol/L ranged from 94.5%to 102.8%with the relative standard deviations(n=5)<4%.The method has good selectivity and high sensitivity,and can be used for the rapid determination of acephate in fruits and vegetables.

关 键 词：荧光光谱法 乙酰甲胺磷 CdSe/ZnS量子点 

分 类 号：O657.39[理学—分析化学]