DBP和TPP联合暴露对斑马鱼胚胎发育的影响  

Effect of Combined Exposure to DBP and TPP on the Development of Zebrafish (Danio rerio) Embryos

作　　者：张明君 户沙沙 姜伟伟 李艳[1] 孙桂金[1] ZHANG Ming-jun;HU Sha-sha;JIANG Wei-wei(School of Food Science and Engineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan,Shandong 250000)

机构地区：[1]齐鲁工业大学(山东省科学院)食品科学与工程学院,山东济南250000

出　　处：《安徽农业科学》2021年第20期105-107,119,共4页Journal of Anhui Agricultural Sciences

基　　金：国家级大学生创新创业训练计划项目(201910431048)。

摘　　要：[目的]研究邻苯二甲酸二丁酯(DBP)和磷酸三苯酯(TPP)单独及联合暴露对斑马鱼胚胎发育的影响。[方法]将受精后4 hpf(hour post-fertilization, hpf)斑马鱼胚胎分别暴露于DBP(0、0.5和1.0 mg/L)、TPP(0和0.5 mg/L)和TPP+DBP(0.5 mg/L+0.5 mg/L和0.5 mg/L+1.0 mg/L),统计72 hpf胚胎孵化率以及96 hpf仔鱼存活率、孵化率、畸形率和体长。[结果]DBP和TPP单独及联合暴露引起斑马鱼卵黄囊肿、心脏畸形、脊柱弯曲和尾巴畸形,导致斑马鱼孵化率和存活率下降、畸形率增加和体长缩短。[结论]DBP和TPP联合暴露对斑马鱼存活率、畸形率和体长影响存在交互作用,对孵化率的影响无交互作用。[Objective]To study the effect of individual and combined exposure to dinbutyl phthalate(DBP) and triphenyl phosphate(TPP) on the development of zebrafish embryos.[Method]Zebrafish embryos at 4 hpf(hour post-fertilization, hpf) were exposed to DBP(0,0.5 mg/L and 1.0 mg/L),TPP(0 and 0.5 mg/L) and TPP+DBP(0.5 mg/L+0.5 mg/L and 0.5 mg/L+1.0 mg/L).The hatching rate of zebrafish embryos was recorded at 72 hpf, and the survival rate, hatching rate, malformation rate and body length of zebrafish larvae were recorded at 96 hpf.[Result]Exposure to individual and combined of DBP and TPP resulted in obvoius malformations in zebrafish, including yolk-sac edema, cardiac deformities, spinal curvature and tail malformation, decreased hatching and survival rates, increased malformation rate and reduced body length in zebrafish embryos.[Conclusion]It has an interaction effect on the survival rate, malformation rate and body length of zebrafish, and no interaction on the hatching rate of zebrafish.

关 键 词：DBP TPP 联合暴露 斑马鱼胚胎 发育 

分 类 号：S917.4[农业科学—水产科学]