黄连水提液对腐皮镰孢菌抑制效果研究  

Study on Inhibitory Effect of the Water Extract of Coptis chinensis on Fusarium solani

作　　者：贾正燕 王昌梅[1,2] 张啸 赵兴玲 吴凯[1,2] 尹芳 杨斌[1] 梁承月 张无敌[1,2] JIA Zheng-yan;WANG Chang-mei;ZHANG Xiao(Yunnan Normal University,Kunming,Yunnan 650500;Jilin Dongsheng Biomass Energy Engineering Research Institute,Tonghua,Jilin 134118;Yunnan Kangyi Sanqi Planting Co.,Ltd.,Kunming,Yunnan 650032)

机构地区：[1]云南师范大学,云南昆明650500 [2]吉林东晟生物质能工程研究院,吉林通化134118 [3]云南康艺三七种植有限公司,云南昆明650032

出　　处：《安徽农业科学》2021年第20期195-197,209,共4页Journal of Anhui Agricultural Sciences

基　　金：云南省万人计划产业技术领军人才项目(20191096);吉林省科技发展计划项目(20200402099NC,20200403010SF);云南省国际科技合作专项(202003AF140001);昆明市国际科技合作基地、云南省农村能源工程重点实验室基金联合项目。

摘　　要：[目的]探究黄连水提液对腐皮镰孢菌(Fusarium solani)的抑菌效果,为深入研究中药对植物根腐病防治提供科学参考。[方法]将黄连剪成0.5 cm左右的小段,称取10 g黄连,加入150mL无菌水煮沸,小火熬30 min,提取其有效成分,得黄连水提液。采用二倍稀释法和菌丝生长速率法测定黄连水提液对腐皮镰孢菌的最小抑菌浓度及最适抑菌量。[结果]1g/mL黄连水提液稀释后对腐皮镰孢菌的抑菌效果较差,4 d平均抑菌率不到20%,最小抑菌浓度为16 mg/mL;1g/mL的黄连水提液增加用量后对腐皮镰孢菌的抑制效果明显增加,最适用量为0.3mL,此时最大抑菌率可达76.56%,最大平均抑菌率达57%。[结论]不同浓度及不同用量黄连水提液对腐皮镰孢菌的菌丝生长具有显著影响,最小抑菌浓度为16 mg/mL,最适抑菌量为0.3mL。[Objective]To investigate the antibacterial effect of water extract of C.chinensis on F.solani,and to provide scientific reference for further research on the prevention and treatment of plant root rot.[Method]C.chinensis was cut into small pieces about 0.5 cm, weighed 10 g of C.chinensis,added 150 mL sterile water to boil for 30 min at low fire, and the effective components were extracted to obtain water extract of C.chinensis.The minimum inhibitory concentration and the optimal inhibitory amount of water extract of C.chinensis against F.solani were determined by double dilution method and mycelial growth rate method.[Result]The antibacterial effect of 1 g/mL water extract of C.chinensis on F.solani was poor.The average antibacterial rate of 4 d was less than 20%,and the MIC value was 16 mg/mL.When the dosage of water extract of 1 g/mL of C.chinensis was increased, the inhibition effect on F.solani was significantly increased.The most suitable dosage was 0.3 mL,and the maximum and average inhibition rate reached 76.56% and 57% at this time.[Conclusion]The different concentrations and dosages of water extract of C.chinensis have significant effects on the mycelial growth of F.solani.The MIC is 16 mg/mL,and the optimal bacteriostatic amount is 0.3 mL.

关 键 词：根腐病 腐皮镰孢菌 黄连水提液 最小抑菌浓度 最适抑菌量 

分 类 号：R285[医药卫生—中药学]