连作与非连作苎麻根际微生物的多样性研究  

Diversity of Microorganism in the Rhizosphere of Continuous Cropping and Non-continuous Cropping on Ramie (Boehmeria nivea(L.) Gaud.)

作　　者：白雪花 陈晓蓉 陈新范 王延周[1] 刘头明[1] 唐守伟[1] 朱四元[1] BAI Xuehua;CHEN Xiaorong;CHEN Xinfan;WANG Yanzhou;LIU Touming;TANG Shouwei;ZHU Siyuan(・Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,Hunan 410205,China;Jiangxi Selenium Industry Research Institute,yichun,Jiangxi 336000,China;・Yiyang Planting Technology Promotion Center,Yiyang,Hunan 413000,China)

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410205 [2]江西富硒产业研究院,江西宜春336000 [3]益阳市种植业技术推广中心,湖南益阳413000

出　　处：《中国麻业科学》2021年第5期222-230,共9页Plant Fiber Sciences in China

基　　金：国家自然科学基金项目(31571618,31771734);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-16-E12)。

摘　　要：苎麻是多年生作物,连作多年会导致苎麻败蔸、产量下降,甚至死亡等,严重影响苎麻产业的发展。试验以连作和非连作苎麻根际土壤为研究对象,采用Illumina MiSeq测序平台,对苎麻根际土壤细菌和真菌群落结构进行分析。结果表明,细菌群落结构中厚壁菌门、变形菌门、酸酐菌门、放线菌门和拟杆菌门为苎麻根际土壤细菌的优势菌门,连作苎麻根际土壤主要细菌为厚壁菌门,占细菌总数的93.46%,而非连作苎麻根际土壤主要为厚壁菌门、变形菌门、酸杆菌门,分别占细菌总数的64.60%、13.70%、5.77%;真菌中子囊菌门和担子菌门为苎麻根际土壤的优势菌门,连作与非连作苎麻根际真菌的门类未发生改变,但是各门类的比率发生了改变;主成分分析表明,连作与非连作苎麻根际土壤微生物丰富度有明显不同。综上所述,与非连作苎麻比较,连作苎麻根际细菌和真菌群落结构以及多样性发生了改变,多样性呈现下降的趋势,且真菌群落的含量比率也发生较大的改变,因此,苎麻根际微生物群落结构和多样性的改变可能是导致苎麻连作障碍的主要因子之一。Ramie is a perennial crop,and continuous cropping for years leads to the decline of ramie stump,yield,or even death,which seriously affects the development of ramie industryin China.The bacteria and fungi community structure of ramie rhizospheres were analyzed using Illumina MiSeq sequencing platform,based on the continuous cropping and non-continuous cropping ramie rhizosphere soil.The results showed that,the Firmicutes,Proteobacteria,Acidobacteria,Actinobacteria and Bacteroidetes were the dominant bacteria in continuous ramie soil,among them,the Firmicutes was the largest one which accounted for 93.46%of total bacteria.However,Fimicutes,Proteobacteria,Acidobacteria were the dominant bacteria in the non-continuousramie soil,which accounted for 64.60%,13.70%and 5.77%respectively in non-continuous ramie rhizosphere soil.Ascomycota and Basidiomycota were the main fungal phyla.The fungi categories did not change,but the ratio of each category changed between replanted and non-replanted ramie rhizosphere soil.Principal component analysis showed that the microbial abundance was obviously different between replanted and non-replanted ramie rhizosphere soil.In conclusion,compared to the healthy ramie soil,the ramie rhizosphere bacteria and fungi community structure have changed,diversity presents the downward trend in the continuous cropping ramie.Therefore,ramie rhizosphere microbial community structure and the change of diversity may be main factors lead to ramie continuous cropping obstacle.

关 键 词：苎麻连作 微生物群落 高通量测序 多样性 

分 类 号：S563.1[农业科学—作物学]