基于银岛薄膜SERS基底的Tau蛋白生物传感器  

Tau protein biosensor based on SERS substrate ofthin silver island film

作　　者：聂倩 张青青 白忠臣[2,3] 张正平 NIE Qian;ZHANG Qingqing;BAI Zhongchen;ZHANG Zhengping(College of Big Data and Information Engineering,Guizhou University,Guiyang 550025,China;Key Laboratory of Photoelectric Technology and Application,Guizhou University,Guiyang 550025,China;College of Medicine,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学大数据与信息工程学院,贵州贵阳550025 [2]贵州大学贵州省光电子技术及应用重点实验室,贵州贵阳550025 [3]贵州大学医学院,贵州贵阳550025

出　　处：《传感器与微系统》2021年第11期17-20,24,共5页Transducer and Microsystem Technologies

基　　金：国家自然科学基金资助项目(61865002,62065002);贵州省科技计划黔科合平台项目([2019]5650);黔科合平台人才项目([2018]5616)。

摘　　要：本文制备了银岛薄膜基底,并利用表面增强拉曼散射(SERS)效应构建了检测Tau蛋白的生物传感器。采用旋涂法制备聚醋酸乙烯醇(PVA)/AgNO 3复合薄膜;然后,通过热还原法制备了具有银岛结构薄膜的SERS基底。使用三聚氰胺作为探针分子,结果表明:基底的SERS增强因子可以达到1149;此外,利用SERS基底检测Tau蛋白,其检测限可达为1 mg/L;且在10~100 mg/L范围内,Tau蛋白浓度与拉曼强度具有线性的“量-效”关系。由于传感器的良好稳定性和低廉加工成本,在阿尔茨海默氏病的早期筛查和诊断等方面具有较好的应用潜力。Surface enhanced Raman scattering(SERS)substrate of thin silver island film is fabricated and Tau protein biosensor is constructed on this substrate.Polyvinyl acetate(PVA)/AgNO 3 composite films are prepared by a spin-coating method.Then,SERS substrate with silver island structure films are prepared by a thermal reduction method.Using melamine as probe molecules,the results show that the SERS enhancement factor of the substrate can reach 1149.In addition,the SERS substrate is used to detect Tau protein,and for detecting limit is up to 1 mg/L.In the concentration range of 10~100 mg/L,Tau protein concentration and Raman strength has a linear“dose-effect”relationship.Because of its good stability and low processing cost,this sensor has a good application potential in early screening and diagnosis of Alzheimer’s disease.

关 键 词：生物传感器 表面增强拉曼散射 TAU蛋白 银岛薄膜 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] O436[自动化与计算机技术—控制科学与工程]