小鼠妊娠疟疾对子代鼠免疫器官T细胞的影响  

Effect of pregnancy malaria on T cells in immune organ of offspring mice

作　　者：陶莉 张培艺[2] 胡瑞 张璐 李春草 方强 夏惠[2] 陶志勇[3] Tao Li;Zhang Peiyi;Hu Rui;Zhang Lu;Li Chuncao;Fang Qiang;Xia Hui;Tao Zhiyong(Shandong Zaozhuang College Hospital,Zaozhuang 277160,China;Department of Microbiology and Parasitology,Bengbu Medical College,Bengbu 233030,China;Anhui Key Laboratory of Infection and Immunity,Bengbu Medical College,Bengbu 233030,China)

机构地区：[1]山东枣庄学院校医院,277160 [2]蚌埠医学院病原生物学教研室,安徽蚌埠233030 [3]蚌埠医学院感染与免疫省级重点实验室,安徽蚌埠233030

出　　处：《中华地方病学杂志》2021年第10期802-807,共6页Chinese Journal of Endemiology

基　　金：安徽省高等学校科研创新平台团队项目(2016-40);蚌埠市-蚌埠医学院联合科技攻关项目(BYLK201828)。

摘　　要：目的观察妊娠期母鼠感染疟原虫对子代鼠细胞免疫功能的影响,并研究胚胎期暴露疟原虫抗原的小鼠感染疟原虫后细胞免疫应答反应的变化。方法选择清洁级成年昆明小鼠,雌雄小鼠合笼,将妊娠第14天的孕鼠按照随机数字表法分为实验组(n=5)和对照组(n=5),实验组每只小鼠经腹腔接种1×10^(6)个感染伯氏疟原虫(Plasmodium berghei,P.b)的红细胞,对照组注射相同体积的生理盐水,孕鼠分娩后得到子代新生鼠(0、1、3、5 d),继续饲养4周,得到4周龄子代鼠。流式细胞术检测子代新生鼠(0、1、3、5 d)及4周龄子代鼠胸腺、脾脏CD4/CD8 T细胞亚群的变化;给予两组4周龄子代鼠腹腔接种P.b 1×10^(6)个/只,于第3天检测其胸腺、脾脏CD4/CD8 T细胞亚群的变化,并采用体外给予P.b抗原或有丝分裂原[刀豆蛋白A(ConA)]刺激方法检测两组子代鼠脾脏细胞对P.b抗原刺激的免疫反应。结果与对照组比较,实验组子代新生鼠(0、1、3、5 d)胸腺、脾脏中CD3^(+)CD4^(+)CD8^(-)T细胞的比例均较高(P均<0.05),而在胸腺中CD3^(+)CD4^(-)CD8^(+)T细胞的比例均较低(P均<0.05);与对照组比较,实验组4周龄子代鼠胸腺、脾脏中CD3^(+)CD4^(+)CD8^(-)T细胞的比例均较高(P均<0.05),而CD3^(+)CD4^(-)CD8^(+)T细胞的比例均较低(P均<0.05);4周龄子代鼠感染P.b后,与对照组比较,实验组胸腺、脾脏中CD3^(+)CD4^(+)CD8^(-)T细胞的比例均较高(P均<0.01),而CD3^(+)CD4^(-)CD8^(+)T细胞的比例均较低(P均<0.05)。两组4周龄子代鼠脾脏细胞,体外给予P.b抗原或有丝分裂原ConA刺激后,实验组CD3^(+)CD4^(+)CD8^(-)T细胞、CD3^(+)CD4^(-)CD8^(+)T细胞比例与对照组比较差异均无统计学意义(P均>0.05)。结论妊娠期母鼠感染P.b可明显影响子代鼠胸腺和脾脏CD4/CD8 T细胞亚群的比例;并改变子代鼠对再感染P.b的细胞免疫应答反应。Objective To observe the influence of pregnant mice having malaria on T cell function of offspring mice,and to study the changes of cellular immune response in offspring mice exposed to malaria infection in uterus.Methods Adult Kunming mice of clean grade were selected after mating,on the 14th day of pregnancy,pregnant mice were randomize assigned into experimental group(n=5)and control group(n=5)according to the method of random number table.Each mouse in the experimental group was intraperitoneally inoculated with 1×10^(6) red blood cells infected with Plasmodium berghei(P.b),and same volume of normal saline was given to control group.After birth,the changes of CD4/CD8 T cell subsets in their thymuses and spleens of the two group neonatal mice were analyzed by flow cytometry at day 0,1,3,5 and 4^(-)week-old.Then the 4^(-)weeks-old neonatal mice were intraperitoneally inoculated with 1×10^(6)P.b.On the third day,the changes of CD4/CD8 T cells subsets in their thymuses and spleens were observed,respectively,and the immune response of spleen cells stimulated by P.b antigen or mitogen[concanavalin A(Con A)]was detected.Results Compared with the control group,the proportions of CD3^(+)CD4^(+)CD8^(-)T cells in thymus and spleen of the offspring of the experimental group(0,1,3,5 days)were higher(P<0.05),while the proportions of CD3^(+)CD4^(-)CD8^(+)T cells in thymus were lower(P<0.05).For 4^(-)week-old offspring and after infection of P.b,the proportions of CD3^(+)CD4^(+)CD8^(-)T cells in thymus and spleen of the experimental group were both significantly higher than those of control group(P<0.05),in contrast,the proportions of CD3^(+)CD4^(-)CD8^(+)T cells in thymus and spleen were both significantly lower than those of control group(P<0.05).The spleen cells of 4^(-)week-old mice were stimulated by P.b antigen or mitogen ConA in vitro,compared with the control group,there were no significant differences in the proportions of CD3^(+)CD4^(+)CD8^(-)T cells and CD3^(+)CD4^(-)CD8^(+)T cells in the experimental group(P>0.

关 键 词：疟疾 伯氏疟原虫 细胞免疫 T细胞 

分 类 号：R531.3[医药卫生—内科学]