宫腔粘连子宫内膜组织中E-cadherin、MMP-9及PDGF-AA的表达水平及临床意义  

Expression level and clinical significance of E-cadherin,MMP-9 and PDGF-AA in endometrial tissue of intrauterine adhesions

作　　者：伍雯莹 丁丽君 谢环[3] WU Wenying;DING Lijun;XIE Huan(Department of Women′s Health Care,Huangshi Municipal Maternal and Child Health Care Hospital,Huangshi,Hubei 435000,China;Department of Obstetrics,Huangshi Municipal Maternal and Child Health Care Hospital,Huangshi,Hubei 435000,China;Department of Obstetrics and Gynecology,Yichang Municipal Second People′s Hospital/Second People′s Hospital of Three Gorge University,Yichang,Hubei 443000,China)

机构地区：[1]湖北省黄石市妇幼保健院妇女保健科,湖北黄石435000 [2]湖北省黄石市妇幼保健院产科,湖北黄石435000 [3]湖北省宜昌市第二人民医院/三峡大学第二人民医院妇产科,湖北宜昌443000

出　　处：《国际检验医学杂志》2021年第21期2561-2566,共6页International Journal of Laboratory Medicine

基　　金：湖北省卫生健康委联合基金项目(WJ2019H524)。

摘　　要：目的探讨宫腔粘连子宫内膜组织中E-钙黏蛋白(E-cadherin)、基质金属蛋白酶-9(MMP-9)、血小板源性生长因子-AA(PDGF-AA)的表达特点,分析其与宫腔粘连患者临床特征、疗效和预后的关系。方法选择2015年6月至2018年7月湖北省黄石市妇幼保健院妇女保健科收治的86例宫腔粘连患者(宫腔粘连组)和51例输卵管性不孕患者(对照组)。免疫组化检测所有患者子宫内膜组织中E-cadherin、MMP-9及PDGF-AA表达情况,追踪宫腔粘连患者术后2年妊娠情况。分析E-cadherin、MMP-9及PDGF-AA表达与宫腔粘连患者临床特征、疗效和预后的关系。结果宫腔粘连组患者子宫内膜组织中MMP-9、PDGF-AA阳性表达率高于对照组(P<0.05),E-cadherin阳性表达率低于对照组(P<0.05)。Nasr分级中重度、输卵管开口不可见、闭经、治疗无效的宫腔粘连患者的MMP-9、PDGF-AA阳性表达率均高于Nasr分级轻度、输卵管开口单侧或双侧可见、无闭经、治疗有效的宫腔粘连患者(P<0.05),E-cadherin阳性表达率低于Nasr分级轻度、输卵管开口单侧或双侧可见、无闭经、治疗有效的宫腔粘连患者(P<0.05)。膜性宫腔粘连患者的E-cadherin阳性表达率高于纤维性和肌性粘连患者(P<0.05)。MMP-9、PDGF-AA表达与Nasr分级呈正相关(r=0.623、0.513,P<0.05),E-cadherin表达与Nasr分级呈负相关(r=-0.569,P<0.05)。MMP-9、PDGF-AA阳性表达者妊娠率低于MMP-9、PDGF-AA阴性表达者(P<0.05),E-cadherin阳性表达者妊娠率高于E-cadherin阴性表达者(P<0.05)。结论宫腔粘连子宫内膜组织中MMP-9、PDGF-AA阳性表达增高,E-cadherin阳性表达降低,E-cadherin、MMP-9、PDGF-AA表达与宫腔粘连病情严重程度、疗效及预后均有关,可作为宫腔粘连预后评估的潜在生物学指标。Objective To investigate the expression characteristics of E-cadherin,matrix metalloproteinase-9(MMP-9)and platelet derived growth factors-AA(PDGF-AA)in endometrial tissue of intrauterine adhesions,and to analyze their relationship with clinical characteristics,efficacy and prognosis.Methods Eighty-six patients with intrauterine adhesions(intrauterine adhesions group)and 51 patients with tubal infertility(control group)treated in the Huangshi Municipal Maternal and Child Health Care Hospital from June 2015 to July 2018 were selected.The immunohistochemistry was used to detect the expression of E-cadherin,MMP-9 and PDGF-AA in endometrial tissues,and the pregnancy status of the patients with intrauterine adhesions was tracked during postoperative 2 years.The relationship between E-cadherin,MMP-9 and PDGF-AA expression with the clinical characteristics,efficacy and prognosis of the patients with intrauterine adhesions was analyzed.Results The expression positive rates of MMP-9 and PDGF-AA in endometrial tissues of the intrauterine adhesion group were higher than those of the control group(P<0.05)and the positive expression rate of E-cadherin was lower than that of the control group(P<0.05).The positive expression rates of MMP-9 and PDGF-AA in the patients with moderate to severe Nasr grade,tubal openings invisible,amenorrhea and treatment failure patients were higher than those in the patients with mild Nasr grade,unilateral or bilateral tubal opening visible,no amenorrhea and treatment effect patients(P<0.05),and the E-cadherin positive expression rate were lower than that in the patients with mild Nasr grade,unilateral or bilateral tubal openings visible,without amenorrhea and treatment effect patients(P<0.05).The positive expression rate of E-cadherin in the patients with membranous intrauterine adhesions was higher than that in the patients with fibrotic and muscular adhesions(P<0.05).The expressions of MMP-9 and PDGF-AA were positively correlated with the Nasr grade(r=0.623,0.513,P<0.05),while E-cadherin was ne

关 键 词：宫腔粘连 子宫内膜 E-钙黏蛋白 基质金属蛋白酶-9 血小板源性生长因子-AA 

分 类 号：R711.74[医药卫生—妇产科学]