两株副猪嗜血杆菌辽宁分离株sRNA64和sRNA145的转录比较分析  

Transcriptional Comparative Analysis of Two Liaoning Isolates of Haemophilus parasuis sRNA64 and sRNA145

作　　者：贾永超 周媛媛 严平 郭营 王临喜 尹荣兰[2] 郭忠博 王静宜 王超 尹荣焕[1] JIA Yong-chao;ZHOU Yuan-yuan;YAN Ping;GUO Ying;WANG Lin-xi;YIN Rong-lan;GUO Zhong-bo;WANG Jing-yi;WANG Chao;YIN Rong-huan(Key Laboratory of Livestock Infectious Diseases in Northeast China,Ministry of Education,College of Animal Science and Veterinary Medicine,Shenyang Agricultural University,Shenyang,Liaoning,110866,China;Jilin Provincial Academy of Animal Husbandry and Veterinary Medicine,Changchun,Jilin,130062,China;Liaoning Agricultural College,Yingkou,Liaoning,115009,China)

机构地区：[1]东北畜禽疫病研究教育部重点实验室,沈阳农业大学畜牧兽医学院,辽宁沈阳110866 [2]吉林省畜牧兽医科学研究院,吉林长春130062 [3]辽宁农业职业技术学院,辽宁营口115009

出　　处：《动物医学进展》2021年第11期6-12,共7页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(32172861);辽宁省自然科学基金项目(2021-MS-221);辽宁省教育厅科学研究项目(LSNJC201917);辽宁省人兽共患病研究工程实验室项目(01072917001)。

摘　　要：为研究副猪嗜血杆菌(Haemophilus parasuis,HPS)相关sRNA(small RNA)的调控机制,以复苏培养后的2株HPS辽宁分离菌株为研究对象,通过实时荧光定量PCR对体外不同生长时期以及感染PK-15细胞后sRNA64和sRNA145的转录水平进行了分析。结果表明,体外生长过程中sRNA64、sRNA145分别在强、弱毒株中差异表达,2株HPS感染细胞后sRNA64均差异表达,而sRNA145仅在强毒株中差异表达。另外,sRNA64、sRNA145分别在强、弱毒株体外生长后期达到转录高峰,感染细胞后sRNA64在强、弱毒株感染早期达到转录高峰,而sRNA145仅在强毒株感染细胞后期达到转录高峰。说明sRNA64和sRNA145作为差异表达基因对调控HPS发挥着作用,研究结果为sRNA在不同毒力HPS致病机制中的进一步研究提供了基础数据。In order to study the regulation mechanism of small RNA(sRNA)related to Haemophilus parasuis,two H.parasuis strains isolated from Liaoning were used as the research objects.The transcription levels of sRNA64 and sRNA145 at different growth periods in vitro and after infection of PK-15 cells were analyzed by real-time quantitative PCR(RT-qPCR).The results showed that sRNA64 and sRNA145 were differentially expressed in the highly virulent strain and non-virulent strain during in vitro growth,and sRNA64 was differentially expressed after the two H.parasuis strains infected cells,while sRNA145 was only differentially expressed in the highly virulent strain.In addition,sRNA64 and sRNA145 reached the transcription peak in the late growth period of the highly virulent strain and non-virulent strain respectively.After infection of cells,sRNA64 reached the transcription peak in the early stage of infection of the highly virulent strain and non-virulent strain,while sRNA145 only reached the transcription peak in the late stage of the infection of the highly virulent strain.This shows that sRNA64 and sRNA145 as differentially expressed genes play a role in regulating H.parasuis,and this result provides basic data for further research on sRNA in the pathogenic mechanism of H.parasuis with different virulence.

关 键 词：副猪嗜血杆菌 SRNA 细胞感染 转录比较分析 

分 类 号：S852.612[农业科学—基础兽医学] S858.28[农业科学—兽医学]