4G手机射频电磁辐射阴囊暴露对成年大鼠生精能力和血-睾屏障的影响  

Exposure of the scrotum to 4G cellphone radiofrequency electromagnetic radiation affects the spermatogenesis and blood-testis barrier of adult male rats

作　　者：程庆[1] 禹刚 王刚 白志明[1] CHENG Qing;YU Gang;WANG Gang;BAI Zhi-ming(Department of Urology,Haikou Hospital Affiliated to Xiangya School of Medicine,Central South University,Haikou,Hainan 51020S,China;Department of Urology,Peoples Hospital of Wuhan University,Wuhan,Hubei 430060,China)

机构地区：[1]中南大学湘雅医学院附属海口医院泌尿外科,海南海口570208 [2]武汉大学人民医院泌尿外科,湖北武汉430060

出　　处：《中华男科学杂志》2021年第8期680-684,共5页National Journal of Andrology

基　　金：海南省重点研发计划(社发方向)资助(ZDYF2016137)。

摘　　要：目的:探讨4G手机射频电磁辐射阴囊暴露对成年大鼠睾丸的直接影响。方法:30只SD大鼠随机分为对照组及暴露组,建立阴囊局部暴露模型,暴露组接受6 h/d的辐射,共150 d。分析精液质量和生精上皮变化;检测抗氧化酶-过氧化物水平及血-睾屏障蛋白的表达。结果:与对照组相比,暴露组精子浓度(6.39±0.82 vs 4.74±0.87)(×10^(7)/ml)、活率(62.11±8.82 vs 41.44±7.33)(%)、总活力(55.71±7.39 vs 36.22±6.36)(%)及正常形态精子百分率(84.89±5.11 vs 70.78±8.11)(%)明显降低(P<0.05);暴露组Johnsen评分明显减低(8.38±0.98 vs 6.11±1.56,P<0.05),Coentino评分明显增高(1.36±0.21 vs 1.81±0.34,P<0.05);暴露组MDA水平明显上升(P<0.05),SOD、GSH及CAT水平明显下降(P<0.05);暴露组Occludin、ZO-1、CAR及N-Cadherin水平显著降低(P<0.05)。结论:4G手机射频电磁辐射阴囊局部暴露可直接导致成年睾丸损伤、血-睾屏障紊乱及精子质量下降,造成大鼠生育力下降。Objective:To investigate the direct impact of exposure of the scrotum to 4 G cellphone radiofrequency electromagnetic radiation(RF-EMR)on the testis of adult rats.Methods:We equally randomized 30 adult male SD rats into a control and an exposure group,the latter exposed to 4 G cellphone RF-EMR 6 hours a day for 150 days.Then,we analyzed the changes in semen quality and seminiferous epithelia,and measured the levels antioxidant enzyme-peroxide and the expressions of blood-testis barrier proteins in the testis of the rats.Results:Compared with the controls,the rats in the exposure group showed significantly decreased sperm concentration([6.39±0.82]vs[4.74±0.87]×10^(7)/ml,P<0.05),viability([62.11±8.82]%vs[41.44±7.33]%,P<0.05),motility([55.71±7.39]vs[36.22±6.36]%,P<0.05),the percentage of morphologically normal sperm([84.89±5.11]%vs[70.78±8.11]%,P<0.05)and Johnsen’s score(8.38±0.98 vs 6.11±1.56,P<0.05),increased Coentino’s score(1.36±0.21 vs 1.81±0.34,P<0.05)and MDA level(P<0.05),reduced levels of SOD,GSH and CAT(P<0.05),and down-regulated expressions of occludin,ZO-1,CAR and N-Cadherin(P<0.05).Conclusion:Exposure of the scrotum to 4 G cellphone RF-EMR directly causes injury of the testis,disorder of the blood-testis barrier,reduction of semen quality and consequently decline in the fertility of male rats.

关 键 词：4G手机 射频电磁辐射 精液质量 血睾屏障 

分 类 号：R698.2[医药卫生—泌尿科学]