拮抗串珠镰刀菌并降解伏马毒素菌株的筛选及降解特性研究  

Study on screening and degradation characterization of a strain antagonizing Fusarium moniliforme and degrading fumonisins

作　　者：伍常菁 张丽 鲁京昆 贾师奇 李皓 郭晓军[1,2] WU Chang-jing;ZHANG Li;LU Jing-kun;JIA Shi-qi;LI Hao;GUO Xiao-jun

机构地区：[1]河北农业大学生命科学学院,河北保定071000 [2]河北省饲用微生物技术创新中心,河北保定071000

出　　处：《饲料研究》2021年第17期56-59,共4页Feed Research

基　　金：河北省自然科学基金(项目编号:C2019204284);河北省重点研发计划农业高质量发展关键共性技术攻关专项(项目编号:21322906D);大中学生科技创新能力培育专项(项目编号:2021H060504)。

摘　　要：为筛选能高效降解伏马毒素,并拮抗串珠镰刀菌的菌株,试验通过十字划线法和平板扩散法进行串珠镰刀菌拮抗菌的初筛,通过酶联免疫吸附法进行伏马毒素降解菌的复筛,并探究了菌株在不同时间、温度、pH值条件下最适降解条件。结果表明,筛选到1株对伏马毒素有强降解作用的拮抗串珠镰刀菌菌株R3-7,降解率为98.92%;结合菌落形态、16S rDNA鉴定和生理生化试验,确定该菌株为枯草芽孢杆菌(Bacillus subtilis)。该菌株的最佳降解时间为9 h,最适降解pH值为7.0,最适降解温度为37℃。In order to screen for strains that can efficiently degrade fumonisins and antagonize Fusarium moniliforme,Cross-hatch method and plate diffusion method were used for screened strains antagonized Fusarium moniliforme.Enzyme-linked immunosorbent assay(ELISA)was used for screen strains degrading fumonisins.The degradation characteristics of strains under different time,temperature and pH values were studied.The results showed that the strain R3-7 antagonizing Fusarium moniliforme and have strong degradation of fumonisin was screened,and the degradation rate was 98.92%.Combined with colony morphology,16S rDNA identification,physiological and biochemical experiments,the strain R3-7 was identified as Bacillus subtilis.The optimum degradation time of the strain was 9 h,the optimum degradation pH value was 7.0 and the optimum degradation temperature was 37℃.

关 键 词：伏马毒素 生物降解 串珠镰刀菌 拮抗 枯草芽孢杆菌 

分 类 号：S816.7[农业科学—饲料科学]