高效液相色谱法测定饲料中金霉素的不确定度评价  

Uncertainty evaluation for determination chlortetracycline in feed by high performance liquid chromatography

作　　者：娄迎霞 姚婷 朱晓静 李俊[1] 谷旭[1] LOU Ying-xia;YAO Ting;ZHU Xiao-jing;LI Jun;GU Xu

机构地区：[1]中国农业科学院饲料研究所,北京100081 [2]北京市饲料监察所,北京100107 [3]北京市畜牧总站,北京100107

出　　处：《饲料研究》2021年第17期89-92,共4页Feed Research

基　　金：全国饲料质量安全风险监测计划;国家重点研发计划“水产动物精准营养及其代谢调控机制”。

摘　　要：文章采用高效液相色谱法对饲料中金霉素含量进行测定分析,并对检测过程进行不确定来源分析,计算检测结果的不确定度。结果显示,当饲料中金霉素含量为69.567 mg/kg时,在95%置信区间下,其结果的扩展不确定度为1.792 mg/kg(k=2)。影响检测结果的各不确定度分量中,标准品纯度和标准工作液稀释过程引入的不确定度对结果的影响最大。The article used high performance liquid chromatography to determine and analyze the content of chlortetracycline in feed,and analyzes the source of uncertainty in the detection process,and calculates the uncertainty of the detection result.The results showed that when the content of chlortetracycline was 69.567 mg/kg,the extended uncertainty of the results was 1.792 mg/kg at the 95% confidence interval(k=2).Among the components affecting the test results,the purity of the standard product and the uncertainty introduced by the standard solution preparation process has a greater impact on the results.

关 键 词：饲料 金霉素 高效液相色谱法 不确定度评价 

分 类 号：S816.17[农业科学—饲料科学]